Team:KAIT Japan/Results
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='''Results'''= | ='''Results'''= | ||
- | ==''' | + | =='''Cytoplasmic Region of Tar'''== |
:'''We did PCR so as to get cytoplasmic region of tar.We used primer with restriction.''' | :'''We did PCR so as to get cytoplasmic region of tar.We used primer with restriction.''' | ||
:[[file:KAIT_Japan-Results-Tar1.jpg]] | :[[file:KAIT_Japan-Results-Tar1.jpg]] | ||
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:'''Figure2.the colony having cytoplasmic region of tar ''' | :'''Figure2.the colony having cytoplasmic region of tar ''' | ||
: | : | ||
- | :'''7.After miniprep we did PCR by using VF2 and VR primer''' | + | :'''7.After miniprep,we did PCR by using VF2 and VR primer''' |
:'''8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.''' | :'''8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.''' | ||
:'''cytoplasmic region of tar bp is varied. 900bp~1000bp''' | :'''cytoplasmic region of tar bp is varied. 900bp~1000bp''' | ||
: | : | ||
:[[file:KAIT_Japan-Results-Tar3.jpg]] | :[[file:KAIT_Japan-Results-Tar3.jpg]] | ||
- | :'''Figure3 PCR production by using VF and | + | :'''Figure3.PCR production by using VF and VR''' |
- | ==''' | + | =='''Methylation Site of Tar'''== |
+ | : | ||
+ | :'''We did PCR so as to get methylation site of tar.We used primer with restriction ''' | ||
+ | :[[file:KAIT_Japan-Results-Met1.jpg]] | ||
+ | :'''Figure1.PCR production from BBa_J22010''' | ||
+ | |||
+ | :'''enzyme tag EcoRⅠand PstⅠ We experimented as following. ''' | ||
+ | :'''1.restriction enzyme processing PCR production ''' | ||
+ | :'''2.ligation ''' | ||
+ | :'''3.transformation''' | ||
+ | :'''4.culture on the LB nutrient medium and get colonies.''' | ||
+ | :'''5.colony PCR and numbering colonies.''' | ||
+ | :'''6.We did miniprep 1,8,9,10.this is colony number''' | ||
+ | :[[file:KAIT_Japan-Results-Met2.jpg]] | ||
+ | :'''Figure2. the colony having methylation site of tar''' | ||
+ | : | ||
+ | :'''7.After miniprep,we did PCR by using VF2 and VR primer ''' | ||
+ | :'''8.The part of red boxes production can be seen the band of methylation site of tar. So,These miniprep production are tar parts.''' | ||
+ | :'''methylation site of tar is 690bp''' | ||
+ | :[[File:KAIT_Japan-Results-Met4.jpg]] | ||
+ | :'''Figure3.PCR production by using VF and VR''' |
Latest revision as of 18:15, 26 September 2012
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