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Team:Groningen/Notebook/Wetwork 18September2012
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Tom<br> | Tom<br> | ||
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| - | Colony pcr for the constructs: amilcp + alsT, lycopene + alsT, amilcp + fnr, lycopene + fnr, amilcp + sboA, lycopene + sboA and amilgfp + sboA.<br> | + | Colony pcr with taq polymerase for the constructs: amilcp + alsT, lycopene + alsT, amilcp + fnr, lycopene + fnr, amilcp + sboA, lycopene + sboA and amilgfp + sboA.<br> |
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The pcr products of amilcp + fnr, amilcp + sboA and amilgfp + sboA were succesfull. The constructs with lycopene need a longer elongation time. The primers for alsT are probably causing flaws.<br> | The pcr products of amilcp + fnr, amilcp + sboA and amilgfp + sboA were succesfull. The constructs with lycopene need a longer elongation time. The primers for alsT are probably causing flaws.<br> | ||
| - | <br> | + | <br> |
| + | Arjan | ||
| + | <br><br> | ||
| + | miniprep of psbic3 with alst+amilCP and SboA+cmilCP/amilGFP/lycopene. Restriction control with EcoRI and PstI. All the construct with SboA are good and will be sent tomorrow. | ||
| + | <br> <br> | ||
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
</p><br><br> | </p><br><br> | ||
Latest revision as of 15:15, 26 September 2012
Tom
Colony pcr with taq polymerase for the constructs: amilcp + alsT, lycopene + alsT, amilcp + fnr, lycopene + fnr, amilcp + sboA, lycopene + sboA and amilgfp + sboA.
The pcr products of amilcp + fnr, amilcp + sboA and amilgfp + sboA were succesfull. The constructs with lycopene need a longer elongation time. The primers for alsT are probably causing flaws.
Arjan
miniprep of psbic3 with alst+amilCP and SboA+cmilCP/amilGFP/lycopene. Restriction control with EcoRI and PstI. All the construct with SboA are good and will be sent tomorrow.
Back to notebook
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