Team:SDU-Denmark/geneseq

From 2012.igem.org

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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  3' GACGTC </br></br>
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  3' GACGTC </br></br>
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</p>
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AGGAGGTGTCACCATGCAAACCCCTGAACCCTTTACAGACCTTGAACATGAACCCCACAC
 
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ACCCCTACTGGACCACCACCACAACCCACCACCACAAACCACCACAAAACCTTTGTTCAC
 
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CAGGGTTGTGTCCGGTGTCACCTTTGTTTTATTCTTCTTTGGTTTCGCTATCGTATTCAT
 
<h2>Our plant genes:</h2>
<h2>Our plant genes:</h2>
<p>
<p>
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First primer    Extended    Start codon    Stop codon    Restriction site        Mutagenesis primer
+
 
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1-FFT:
+
<b>1-SST:</b>
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AGGAGGTGTCACCATGCAAACCCCTGAACCCTTTACAGACCTTGAACATGAACCCCACACACCCCTACTGGACCACCACCACAACCCACCACCACAAACCACCACAAAACCTTTGTTCACCAGGGTTGTGTCCGGTGTCACCTTTGTTTTATTCTTCTTTGGTTTCGCTATCGTATTCATTGTTCTCAACCAACAGAACTCTTCTGTTCGTATCGTCACCAATTCGGAGAAATCTTTTATAAGGTATTCGCAGACCGATCGCTTGTCGTGGGAACGGACCGCTTTTCATTTTCAGCCTGCCAAGAATTTTATTTACGATCCAGATGGTCAGTTGTTTCACATGGGCTGGTACCATATGTTCTATCAATACAACCCATACGCACCGGTTTGGGGCAATATGTCATGGGGTCACTCAGTGTCCAAAGACATGATCAACTGGTACGAGCTGCCAGTCGCTATGGTCCCGACCGAATGGTATGATATCGAGGGCGTCTTATCCGGGTCTACCACGGTCCTTCCAAACGGTCAGATCTTTGCATTGTATACTGGGAACGCTAATGATTTTTCCCAATTACAATGCAAAGCTGTACCCGTAAACTTATCTGACCCGCTTCTTATTGAGTGGGTCAAGTATGAGGATAACCCAATCCTGTACACTCCACCAGGGATTGGGTTAAAGGACTATCGGGACCCGTCAACAGTCTGGACAGGTCCCGATGGAAAGCATAGGATGATCATGGGAACTAAACGTGGCAATACAGGCATGGTACTTGTTTACTATACCACTGATTACACGAACTACGAGTTGTTGGATGAGCCGTTGCACTCTGTTCCCAACACCGATATGTGGGAATGCGTCGACTTTTACCCGGTTTCGTTAACCAATGATAGTGCACTTGATATGGCGGCCTATGGGTCGGGTATCAAACACGTTATTAAAGAAAGTTGGGAGGGACATGGAATGGATTGGTATTCAATCGGGACATATGACGCGATAAATGATAAATGGACTCCCGATAACCCGGAACTAGATGTCGGTATCGGGTTACGGTGCGATTACGGGAGGTTTTTTGCATCAAAGAGTCTTTATGACCCATTGAAGAAAAGGAGGATCACTTGGGGTTATGTTGGAGAATCAGATAGTGCTGATCAGGACCTCTCAAGAGGATGGGCTACTGTTTATAATGTTGGAAGAACAATTGTACTAGATAGAAAGACCGGGACCCATTTACTTCATTGGCCCGTTGAGGAAGTCGAGAGTTTGAGATACAACGGTCAGGAGTTTAAAGAGATCAAGCTAGAGCCCGGTTCAATCATTCCACTCGACATAGGCACGGCTACACAGTTGGACATAGTTGCAACATTTGAGGTGGATCAAGCAGCGTTGAACGCGACAAGTGAAACCGATGATATTTATGGTTGCACCACTAGCTTAGGTGCAGCCCAAAGGGGAAGTTTGGGACCATTTGGTCTTGCGGTTCTAGCCGATGGAACCCTTTCTGAGTTAACTCCGGTTTATTTCTATATAGCTAAAAAGGCAGATGGAGGTGTGTCGACACATTTTTGTACCGATAAGCTAAGGTCATCACTAGATTATGATGGGGAGAGAGTGGTGTATGGGGGCACTGTTCCTGTGTTAGATGATGAAGAACTCACAATGAGGCTATTGGTGGATCATTCGATAGTGGAGGGGTTTGCGCAAGGAGGAAGGACGGTTATAACATCAAGGGCGTATCCAACAAAAGCGATATACGAACAAGCGAAGCTGTTCTTGTTCAACAACGCCACAGGTACGAGTGTGAAAGCATCTCTCAAGATTTGGCAAATGGCTTCTGCACCAATTCATCAATACCCTTTTTAATTACCGGCTATCGCTATCCTTTTTGTTATTG
+
 
 +
<b>1-FFT:</b>
 +
 

Latest revision as of 14:58, 26 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID



Standard restriction sites:


EcoRI:          5' GAATTC
                      3' CTTAAG

XbaI:            5' T*CTAGA
                      3' AGATC*T

SpeI:             5' A*CTAGT
                      3' TGATC*A

PstI:              5' CTGCAG
                      3' GACGTC

Our plant genes:

1-SST: 1-FFT:

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