Notebook

Our team pushed forward an experiment while making trial and error every day.

Creating parts of Methylation

Creating parts of Tar

Date:9/13 PCR and Colony PCR

Date:9/14 Electrophoretic confirmation

Date:9/15 Restriction enzyme processing

Date:9/16 Electrophoresis of the restriction enzyme processing product and Restriction enzyme processing

Date:9/17 Refinement of DNA and Ligation,Transformation

Date:9/18 Making of a master plate and Preculture,Recultivation

Date:9/19 Colony PCR and Electrophoresis

Date:9/20 Ligation and Transformation

Date:9/21 Inoculation

Date:9/22 Making of a master plate and Colony PCR,Electrophoresis,Preculture

Date:9/23 Electrophoretic confirmation and Electrophoresis of the PCR

Date:9/24 Miniprep and Electrophoresis

Date:9/25 PCR of the miniprep product and Electrophoresis

Creating parts of EnvZ

Creating parts of Azurin

Creating parts of Tar methylation region

Date:8/9 Colony PCR

Date:8/11 The purified DNA

Date:8/13 Confirmed of electrophoresis by PCR product and Ligation of the TA vector

Date:8/14 Transformation

Date:8/25 Miniprep

Date:8/30 Electrophoresis,Refinement of DNA,PCR,Nanodrop

Date:9/1 Miniprep,PCR,Elecrtophoresis

Date:9/2 Meeting

Date:9/5 Miniprep,Nanodrop

Date:9/6 Miniprep,PCR,Electrophoresis

Date:9/10 Colony PCR,Refinement of DNA,c,Miniprep

Date:9/11 Primer design

Date:9/12 Miniprep,Ligation

Date:9/13 Electrophoresis,Refinement of DNA

Date:9/14 Colony PCR

Date:9/15 Ligation

Date:9/16 PCR,Ligation

Date:9/17 PCR,Ligation

Date:9/19 Ligation

Date:9/20 Enzyme inactivation,Ligation

Date:9/22 Transformation,Colony PCR,Electrophoresis,Miniprep

Date:9/23 Colony PCR,Electrophoresis,Streak

Date:9/25 Colony PCR

Creating parts of azurin

Date:8/16 Colony PCR,Ligation

Date:8/18 Colony PCR

Date:8/20 DNA extraction and purification of P.aeruginosa

Date:8/30 Electrophoresis,Refinement of DNA,PCR

Date:9/1 Electrophoresis

Date:9/3 Ligation,Transformation,PCR,Nanodrop