Notebook

Our team pushed forward an experiment while making trial and error every day.

Creating parts of Methylation

Creating parts of Tar

Creating parts of EnvZ

Creating parts of Azurin

Creating parts of Tar methylation region

Date:8/9 Colony PCR

Date:8/11 The purified DNA

Date:8/13 Confirmed of electrophoresis by PCR product and Ligation of the TA vector

Date:8/14 Transformation

Date:8/25 Miniprep

Date:8/30 Electrophoresis,Refinement of DNA,PCR,Nanodrop

Date:9/1 Miniprep,PCR,Elecrtophoresis

Date:9/2 Meeting

Date:9/5 Miniprep,Nanodrop

Date:9/6 Miniprep,PCR,Electrophoresis

Date:9/10 Colony PCR,Refinement of DNA,Ligation,Miniprep

Date:9/11 Primer design

Date:9/12 Miniprep,Ligation

Date:9/13 Electrophoresis,Refinement of DNA

Date:9/14 Colony PCR

Date:9/15 Ligation

Date:9/16 PCR,Ligation

Date:9/17 PCR,Ligation

Date:9/19 Ligation

Date:9/20 Enzyme inactivation,Ligation

Date:9/22 Transformation,Colony PCR,Electrophoresis,Miniprep

Date:9/23 Colony PCR,Electrophoresis,Streak

Date:9/25 Colony PCR

Creating parts of azurin

Date:8/16 Colony PCR,Ligation

Date:8/18 Colony PCR

Date:8/20 DNA extraction and purification of P.aeruginosa

Date:8/30 Electrophoresis,Refinement of DNA,PCR

Date:9/1 Electrophoresis

Date:9/3 Ligation,Transformation,PCR,Nanodrop