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Team:LMU-Munich/Data
From 2012.igem.org
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| - | === | + | ===Promoter evaluation=== |
{| "width=100%" style="text-align:center;"| | {| "width=100%" style="text-align:center;"| | ||
| - | |<p align="justify">We | + | |<p align="justify">We evaluated the three promoters for the crust proteins</p> |
| - | |[[File: | + | |[[File:Promotoren.png|200px|link=Team:LMU-Munich/Data/crustpromoters]] |
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| - | ! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/ | + | ! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/crustpromoters]] |
|} | |} | ||
</div> | </div> | ||
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| - | === | + | ===GFP-Sporobead Evaluation=== |
{| "width=100%" style="text-align:center;"| | {| "width=100%" style="text-align:center;"| | ||
| - | |<p align="justify">We | + | |<p align="justify">We fused a GFP to the spore crust with five different constructs in two different genetic ''B. subtilis'' strains and analysed the strength with fluorescence microscopy</p> |
| - | |[[File: | + | |[[File:GFP_spore_data.png|200px|link=Team:LMU-Munich/Data/gfp_spore]] |
|- | |- | ||
| - | ! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/ | + | ! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/gfp_spore]] |
| + | |} | ||
| + | </div> | ||
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| + | <div class="box"> | ||
| + | ===Spore purification=== | ||
| + | {| "width=100%" style="text-align:center;"| | ||
| + | |<p align="justify">We compared different ways of purifying ''Bacillus'' spores from vegetative cells</p> | ||
| + | |[[File:Purification preview.png|200px|link=Team:LMU-Munich/Data/Sporepurification]] | ||
| + | |- | ||
| + | ! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Sporepurification]] | ||
|} | |} | ||
</div> | </div> | ||
| + | |||
Revision as of 12:13, 26 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Data
Here you will find all of our project data.
BacillusBioBrickBox
Evaluation of B. subtilis vectors
Evaluation of B. subtilis vectors in B. subtilis with different inserts. |  |
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Anderson Promoter Evaluation
Evaluation of the Anderson promoters in B. subtilis with the lux operon (luminescence) and the reporter lacZ (β-galactosidase assays). | 
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Constitutive Bacillus Promoters
Evaluation of the constitutive B. subtilis promoters PliaG, Pveg and PlepA with the lux operon (luminescence) and the reporter lacZ (β-galactosidase assays). | 
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Inducible Bacillus Promoters
Evaluation of the inducible B. subtilis promoter PliaI with the lux operon (luminescence) and the reporter lacZ (β-galactosidase assays). | 
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Sporobeads
Promoter evaluation
We evaluated the three promoters for the crust proteins | 
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GFP-Sporobead Evaluation
We fused a GFP to the spore crust with five different constructs in two different genetic B. subtilis strains and analysed the strength with fluorescence microscopy | 200px |
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Spore purification
We compared different ways of purifying Bacillus spores from vegetative cells | 
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GerminationSTOP
Knockouts of germination genes
Germination assy of Bacillus strains with multiple gene knockouts | 
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Suicide Switch
Platereader assay of Suicide switch with lux in the place of MazF with PspoIVB | 
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Inverter
ONPG assay of Inverter with lacZalpha
ONPG assay of Inverter with lacZalpha dependent on Arabinose concentrations | 
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Trashcan
Things that were a pain in the neck!
cgeA and PcgeA
Unexpected restriction sites combined with no activity at all | 
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MazF
The story of trying to clone a toxine from E. coli in E. coli | 
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Xylose-promoters
All constructs involving Xylose-inducible promoter refused cloning or evaluation. | 
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For our big breakthroughs, or to follow specific projects, see the individual project pages:
