Team:Amsterdam/extra/diary
From 2012.igem.org
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- | <h1>Diary</h1> | + | <h1>Lab Diary</h1> |
+ | __NOTOC__ | ||
+ | <html> | ||
+ | <div align='center'> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td colspan='5' align='center'><b>Table of Contents</b></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#12.2F07.2F2012'>12/07/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#11.2F07.2F2012'>11/07/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#10.2F07.2F2012'>10/07/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#09.2F07.2F2012'>09/07/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#06.2F07.2F2012'>06/07/2012</a> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#05.2F07.2F2012'>05/07/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#04.2F07.2F2012'>04/07/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#03.2F07.2F2012'>03/07/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#05.2F06.2F2012'>05/06/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#01.2F06.2F2012'>01/06/2012</a> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#31.2F05.2F2012'>31/05/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#30.2F05.2F2012'>30/05/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#29.2F05.2F2012'>29/05/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#25.2F05.2F2012'>25/05/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#24.2F05.2F2012'>24/05/2012</a> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#23.2F05.2F2012'>23/05/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#16.2F05.2F2012'>16/05/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#14.2F05.2F2012'>14/05/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | <a href='https://2012.igem.org/Team:Amsterdam/extra/diary#11.2F05.2F2012'>11/05/2012</a> | ||
+ | </td> | ||
+ | <td> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div> | ||
+ | </html> | ||
<h4>12/07/2012</h4> | <h4>12/07/2012</h4> | ||
Transformation of the plasmid containing the M.ScaI methyltransferase (pIDTSMART-AMP). | Transformation of the plasmid containing the M.ScaI methyltransferase (pIDTSMART-AMP). | ||
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Digestion of extracted plasmid with NotI and BglII (double digest). | Digestion of extracted plasmid with NotI and BglII (double digest). | ||
- | + | [[File:Amsterdam_lab_diary_1.jpg|300px|border]] | |
Ladder | Ladder | ||
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Pooled all the PCR products for peGFP and gel extract the correct band. Elution in 25µl miliQ water. | Pooled all the PCR products for peGFP and gel extract the correct band. Elution in 25µl miliQ water. | ||
- | + | [[File:Amsterdam_lab_diary_2.jpg|300px|border]] | |
Ladder | Ladder | ||
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Gel electrophoresis of precipitated DNA pET28a+ from 25/05/2012 | Gel electrophoresis of precipitated DNA pET28a+ from 25/05/2012 | ||
- | + | [[File:Amsterdam_lab_diary_3.jpg|300px|border]] | |
Ladder | Ladder | ||
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PCR pET28a+ -> annealing temperature 62˚C. | PCR pET28a+ -> annealing temperature 62˚C. | ||
- | + | [[File:Amsterdam_lab_diary_4.jpg|300px|border]] | |
Ladder | Ladder | ||
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Repeat what was done on 14/05 (PCR Temp 60˚C + gel extraction. | Repeat what was done on 14/05 (PCR Temp 60˚C + gel extraction. | ||
- | + | [[File:Amsterdam_lab_diary_5.jpg|300px|border]] | |
Two products obtained and concentration seems too low. Pfffffff.... Repeat again......And this is only a test, hope it will work better for the actual experiments | Two products obtained and concentration seems too low. Pfffffff.... Repeat again......And this is only a test, hope it will work better for the actual experiments | ||
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<h4>16/05/2012</h4> | <h4>16/05/2012</h4> | ||
- | + | [[File:Amsterdam_lab_diary_6.jpg|300px|border]] | |
pET28a+ (gel extracted) | pET28a+ (gel extracted) | ||
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PCR of pET28a+ and peGFP -> annealing temperature 56˚C. non-specific DNA products obtained for the vector. Increase annealing temperature? Gel extract? | PCR of pET28a+ and peGFP -> annealing temperature 56˚C. non-specific DNA products obtained for the vector. Increase annealing temperature? Gel extract? | ||
- | + | [[File:Amsterdam_lab_diary_7.jpg|300px|border]] | |
Ladder | Ladder | ||
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Gel electrophoresis of the PCR reaction performed on 10/05/2012. Non-specific products obtained. Repeat PCR? Change annealing temperature? | Gel electrophoresis of the PCR reaction performed on 10/05/2012. Non-specific products obtained. Repeat PCR? Change annealing temperature? | ||
- | + | [[File:Amsterdam_lab_diary_8.jpg|300px|border]] | |
Ladder | Ladder |
Latest revision as of 11:46, 26 September 2012