Notebook

Our team pushed forward an experiment while making trial and error every day.

Creating parts of Tar methylation region

Date:8/9

Colony PCR

→Reflection:We Took E.coli too many.You should take less E.coli.

→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles)

Date:8/11

The purified DNA

→Reflection:Band was less.

PCR Product

Date:8/13

Confirmed of electrophoresis by PCR product and Ligation of the TA vector

Date:8/14

Transformation

Date:8/25

Miniprep

Date:9/10

Date:9/11

Primer design

Date:9/12

Miniprep,Ligation

Date:9/13

Electrophoresis,Refinement of DNA

Date:9/14

Colony PCR

Date:9/15

Ligation

Date:9/16

PCR,Ligation

Date:9/17

PCR,Ligation

Date:9/19

Ligation

Date:9/20

Enzyme inactivation,Ligation

Date:9/22

Transformation,Colony PCR,Electrophoresis,Miniprep

Date:9/23 Colony PCR,Electrophoresis,Streak

Date:9/25 Colony PCR

Creating parts of azurin

Date:8/16

Colony PCR

Ligation

Date:8/18

Colony PCR

Date:8/20

DNA extraction and purification of P.aeruginosa