Team:Penn/Notebook/DrugDelivery

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'''Wet Lab'''
'''Wet Lab'''
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We PCR'ed out mCherry from the NAS 151 plasmid. Following this we did a gel purification which resulted in a 30ns/<math>lambda</math> yield.
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We performed PCR to obtain mCherry from the NAS 151 plasmid. Following this we ran a 1% gel and purified the product which resulted in a 30ns/λ yield.
== '''06/12/2012''' ==
== '''06/12/2012''' ==

Revision as of 19:47, 26 June 2012

Contents

06/07/2012

Wet Lab

Today we transformed both versions of cph8 from 2012 Distribution.

Dry Lab

We also placed order for the following new BioBricks:

  • BBa_K207001 - PhyB-DBD Fusion
  • BBa_K422012 - Pif3 (Aar1 C part)
  • BBa_K422013 - PhyB (Aar1 C part)
  • BBa_K592006 - pFixK2
  • BBa_K592004 - YF1
  • BBa_K592005 - FixJ
  • BBa_K592000 - Cph8
  • BBa_K365000 - Pif3

In addition, we brainstormed and developed a new idea to use the PYP/GCN system to activate a gene.

06/08/2012

Wet Lab

No colonies appeared in the transformation of both cph8 a/b. We suspect that the failure of the transformatiosns were due to bad sequences.

06/11/2012

Wet Lab

We performed PCR to obtain mCherry from the NAS 151 plasmid. Following this we ran a 1% gel and purified the product which resulted in a 30ns/λ yield.

06/12/2012

Wet Lab

We transformed pCyA and HO1

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