Why?
From our work on LOV2 photoactivation we might be able to predict the percentage of LOV2 domains in our LovTAP-VP16, even though this value might be affected by the fact of having both ends fused to other domains: TrpR and VP16.
92% of photoactive LOV2 has it's Jα-helix undocked (Yao et al, 2008).
Eitoku et al (2005) say it takes 2 ms for the J&alpha-helix to undock.