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Team:Slovenia/ModelingPositiveFeedbackLoopSwitchStochastic
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<h2><a name="model">The model</a></h2> | <h2><a name="model">The model</a></h2> | ||
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| + | The basis for the stochastic simulation of the positive feedback loop switch was the following set of reactions that describe the dynamics of the switch: | ||
| + | </p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/0/05/Svn12_m_pfs_stoch1.PNG" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/9/93/Svn12_m_pfs_stoch2.PNG" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/3/3e/Svn12_m_pfs_stoch3.PNG" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/5/54/Svn12_m_pfs_stoch4.PNG" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/6/6e/Svn12_m_pfs_stoch5.PNG" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/7/73/Svn12_mrs_stoh6.PNG" /></p> | ||
| + | <p><img src="https://static.igem.org/mediawiki/2012/e/ea/Svn12_m_pfs_stoch7.PNG" /></p> | ||
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Revision as of 08:15, 26 September 2012
Modeling - positive feedback loop switch
- Deterministic model
- Stochastic model
- C#Sim model
Stochastic model of the positive feedback loop switch
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Stochastic simulation, in agreement with the deterministic analysis, proved that the positive feedback loop switch was more robust than the mutual repressor switch. It exhibited bistability without cooperativity (i.e. cooperativity equal to 1) even for leaky expression that caused the mutual repressor switch to lose bistable behavior. Higher cooperativity further increased the robustness and leaky expression tolerance, allowing for higher expression levels to be reached. |
The model
The basis for the stochastic simulation of the positive feedback loop switch was the following set of reactions that describe the dynamics of the switch:
Simulation results
Next: C#Sim model of the positive feedback loop switch >>
