6th august

1. Sending of the B1 sample to sequencing with two pairs of primers
1. K-274100 Forward and Reverse
2. Plasmid pSB1A2 Forward and Reverse

7th august

1. Liquid culture of B1 in order to prepare a glycerol stock
2. Receipt of new primers
1. 2 Forward
2. 3 Reverse
3. Plasmid Reverse

8th august

Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.

9th august

New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before. Digestion by EcoRI of the plasmid that contains BBa_K274100.