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Team:Paris-Saclay/Project/Notebook
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<li>Sending of the B1 sample to sequencing with two pairs of primers</li> | <li>Sending of the B1 sample to sequencing with two pairs of primers</li> | ||
| - | <li> | + | <ol> |
| + | <li>K-274100 Forward and Reverse</li> | ||
| + | <li>Plasmid pSB1A2 Forward and Reverse</li> | ||
| + | </ol> | ||
| + | </ol></br> | ||
| + | |||
| + | <p><b><i>7th august</i></b></p> | ||
| + | <ol> | ||
| + | <li>Liquid culture of B1 in order to prepare a glycerol stock</li> | ||
| + | <li>Receipt of new primers</li> | ||
<ol> | <ol> | ||
<li>2 Forward</li> | <li>2 Forward</li> | ||
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<li>Plasmid Reverse</li> | <li>Plasmid Reverse</li> | ||
</ol> | </ol> | ||
| - | + | </ol></br> | |
| - | </ol> | + | <p><b><i>8th august</i></b></p> |
| - | < | + | <p>Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid. |
| - | + | <img width="350px" src="https://static.igem.org/mediawiki/2012/c/c3/Week10-2.jpg"/> | |
| - | + | <img width="500px" src="https://static.igem.org/mediawiki/2012/8/8b/Week10-3.jpg"/> | |
| - | + | </p></br> | |
| - | + | <p><b><i>9th august</i></b></p> | |
| - | + | <p>New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before. | |
| - | + | <img width="350px" src="https://2012.igem.org/File:Week10-4.jpg"/> | |
| - | + | Digestion by EcoRI of the plasmid that contains BBa_K274100. | |
| - | + | </p></br> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | </ | + | |
</div> | </div> | ||
</li> | </li> | ||
Latest revision as of 06:46, 26 September 2012
GEMOTE
GEMOTE
-
First WeekOpen or Close
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Second WeekOpen or Close
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Third WeekOpen or Close
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Fourth WeekOpen or Close
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Fifth WeekOpen or Close
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Sixth WeekOpen or Close
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Seventh WeekOpen or Close
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Eighth WeekOpen or Close
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Ninth WeekOpen or Close
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Tenth WeekOpen or Close
6th august
- Sending of the B1 sample to sequencing with two pairs of primers
- K-274100 Forward and Reverse
- Plasmid pSB1A2 Forward and Reverse
7th august
- Liquid culture of B1 in order to prepare a glycerol stock
- Receipt of new primers
- 2 Forward
- 3 Reverse
- Plasmid Reverse
8th august
Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.
9th august
New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before.
Digestion by EcoRI of the plasmid that contains BBa_K274100.
-
Eleventh WeekOpen or Close
-
Thirteenth WeekOpen or Close
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