Team:Paris-Saclay/Project/Notebook

From 2012.igem.org

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[[Category:Team:Paris-Saclay/Project Gemote|5]]
[[Category:Team:Paris-Saclay/Project Gemote|5]]
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<div id="accordeon">
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                             <a href="#">First Week<span class="st-arrow">Open or Close</span></a>
                             <a href="#">First Week<span class="st-arrow">Open or Close</span></a>
                             <div class="st-content">
                             <div class="st-content">
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                                <p>She packed her seven versalia, put her initial into the belt and made herself on the way.</p>
 
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                                <p>When she reached the first hills of the Italic Mountains, she had a last view back on the skyline of her hometown Bookmarksgrove, the headline of Alphabet Village and the subline of her own road, the Line Lane.</p>
 
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                                <p>Even the all-powerful Pointing has no control about the blind texts it is an almost unorthographic life One day however a small line of blind text by the name of Lorem Ipsum decided to leave for the far World of Grammar.</p>
 
                             </div>
                             </div>
                         </li>
                         </li>
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                             <a href="#">Second Week<span class="st-arrow">Open or Close</span></a>
                             <a href="#">Second Week<span class="st-arrow">Open or Close</span></a>
                             <div class="st-content">
                             <div class="st-content">
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                                <p>O my friend - but it is too much for my strength - I sink under the weight of the splendour of these visions!</p>
 
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                                <p>A wonderful serenity has taken possession of my entire soul, like these sweet mornings of spring which I enjoy with my whole heart.</p>
 
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                                <p>I am alone, and feel the charm of existence in this spot, which was created for the bliss of souls like mine.</p>
 
                             </div>
                             </div>
                         </li>
                         </li>
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                             <a href="#">Third Week<span class="st-arrow">Open or Close</span></a>
                             <a href="#">Third Week<span class="st-arrow">Open or Close</span></a>
                             <div class="st-content">
                             <div class="st-content">
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                                <p>The bedding was hardly able to cover it and seemed ready to slide off any moment. His many legs, pitifully thin compared with the size of the rest of him, waved about helplessly as he looked. "What's happened to me?"</p>
 
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                                <p>He thought. It wasn't a dream. His room, a proper human room although a little too small, lay peacefully between its four familiar walls.</p>
 
                             </div>
                             </div>
                         </li>
                         </li>
                         <li>
                         <li>
                             <a href="#">Fourth Week<span class="st-arrow">Open or Close</span></a>
                             <a href="#">Fourth Week<span class="st-arrow">Open or Close</span></a>
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                             <div class="st-content">
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                             <div class="st-content">
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                                <p>It showed a lady fitted out with a fur hat and fur boa who sat upright, raising a heavy fur muff that covered the whole of her lower arm towards the viewer. Gregor then turned to look out the window at the dull weather.</p>
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                                <p>Drops of rain could be heard hitting the pane, which made him feel quite sad.</p>
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                             </div>
                             </div>
                         </li>
                         </li>
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                             <a href="#">Fifth Week<span class="st-arrow">Open or Close</span></a>
                             <a href="#">Fifth Week<span class="st-arrow">Open or Close</span></a>
                             <div class="st-content">
                             <div class="st-content">
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                                <p>It showed a lady fitted out with a fur hat and fur boa who sat upright, raising a heavy fur muff that covered the whole of her lower arm towards the viewer. Gregor then turned to look out the window at the dull weather.</p>
 
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                                <p>Drops of rain could be heard hitting the pane, which made him feel quite sad.</p>
 
                             </div>
                             </div>
                         </li>
                         </li>
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                             <a href="#">Sixth Week<span class="st-arrow">Open or Close</span></a>
                             <a href="#">Sixth Week<span class="st-arrow">Open or Close</span></a>
                             <div class="st-content">
                             <div class="st-content">
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                                <p>O my friend - but it is too much for my strength - I sink under the weight of the splendour of these visions!</p>
 
-
                                <p>A wonderful serenity has taken possession of my entire soul, like these sweet mornings of spring which I enjoy with my whole heart.</p>
 
-
                                <p>I am alone, and feel the charm of existence in this spot, which was created for the bliss of souls like mine.</p>
 
                             </div>
                             </div>
                         </li>
                         </li>
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                             <a href="#">Seventh Week<span class="st-arrow">Open or Close</span></a>
                             <a href="#">Seventh Week<span class="st-arrow">Open or Close</span></a>
                             <div class="st-content">
                             <div class="st-content">
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                                <p>It showed a lady fitted out with a fur hat and fur boa who sat upright, raising a heavy fur muff that covered the whole of her lower arm towards the viewer. Gregor then turned to look out the window at the dull weather.</p>
 
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                                <p>Drops of rain could be heard hitting the pane, which made him feel quite sad.</p>
 
                             </div>
                             </div>
                         </li>
                         </li>
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                             <a href="#">Eighth Week<span class="st-arrow">Open or Close</span></a>
                             <a href="#">Eighth Week<span class="st-arrow">Open or Close</span></a>
                             <div class="st-content">
                             <div class="st-content">
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                                <p>It showed a lady fitted out with a fur hat and fur boa who sat upright, raising a heavy fur muff that covered the whole of her lower arm towards the viewer. Gregor then turned to look out the window at the dull weather.</p>
 
-
                                <p>Drops of rain could be heard hitting the pane, which made him feel quite sad.</p>
 
                             </div>
                             </div>
                         </li>
                         </li>
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                             <a href="#">Ninth Week<span class="st-arrow">Open or Close</span></a>
                             <a href="#">Ninth Week<span class="st-arrow">Open or Close</span></a>
                             <div class="st-content">
                             <div class="st-content">
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                                <p>No one rejects, dislikes, or avoids pleasure itself, because it is pleasure, but because those who do not know how to pursue pleasure rationally encounter consequences that are extremely painful.</p>
 
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                                <p>Nor again is there anyone who loves or pursues or desires to obtain pain of itself, because it is pain, but because occasionally circumstances occur in which toil and pain can procure him some great pleasure.</p>
 
                             </div>
                             </div>
                         </li>
                         </li>
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                             <a href="#">Tenth Week<span class="st-arrow">Open or Close</span></a>
                             <a href="#">Tenth Week<span class="st-arrow">Open or Close</span></a>
                             <div class="st-content">
                             <div class="st-content">
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                                <p>It showed a lady fitted out with a fur hat and fur boa who sat upright, raising a heavy fur muff that covered the whole of her lower arm towards the viewer. Gregor then turned to look out the window at the dull weather.</p>
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                              <p><b><i>6th august</i></b></p>
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                                <p>Drops of rain could be heard hitting the pane, which made him feel quite sad.</p>
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<ol>
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<li>Sending of the B1 sample to sequencing with two pairs of primers</li>
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<ol>
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<li>K-274100 Forward and Reverse</li>
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<li>Plasmid pSB1A2 Forward and Reverse</li>
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</ol>
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</ol></br>
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                              <p><b><i>7th august</i></b></p>
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<ol>
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<li>Liquid culture of B1 in order to prepare a glycerol stock</li>
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<li>Receipt of new primers</li>
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<ol>
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<li>2 Forward</li>
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<li>3 Reverse</li>
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<li>Plasmid Reverse</li>
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</ol>
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</ol></br>
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<p><b><i>8th august</i></b></p>
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<p>Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.
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<img width="350px" src="https://static.igem.org/mediawiki/2012/c/c3/Week10-2.jpg"/>
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<img width="500px" src="https://static.igem.org/mediawiki/2012/8/8b/Week10-3.jpg"/>
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</p></br>
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<p><b><i>9th august</i></b></p>
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<p>New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before.
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<img width="350px" src="https://2012.igem.org/File:Week10-4.jpg"/>
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Digestion by EcoRI of the plasmid that contains BBa_K274100.
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</p></br>
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                            </div>
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                        </li>
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<li>
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                            <a href="#">Eleventh Week<span class="st-arrow">Open or Close</span></a>
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                            <div class="st-content">
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                            </div>
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                        </li>
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<li>
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                            <a href="#">Thirteenth Week<span class="st-arrow">Open or Close</span></a>
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                            <div class="st-content">
                             </div>
                             </div>
                         </li>
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Latest revision as of 06:46, 26 September 2012

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