Team:Paris-Saclay/Project/Notebook
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<img src="http://www.igem-paris-saclay.u-psud.fr/wordpress/wp-content/uploads/2012/07/Aud.jpg" alt="" /> | <img src="http://www.igem-paris-saclay.u-psud.fr/wordpress/wp-content/uploads/2012/07/Aud.jpg" alt="" /> | ||
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<img src="http://www.ecole-adn.fr/uploads/2011/09/Probiotics-300x300.jpg" alt="" /> | <img src="http://www.ecole-adn.fr/uploads/2011/09/Probiotics-300x300.jpg" alt="" /> | ||
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{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} | ||
- | [[Category:Team:Paris-Saclay/Project Gemote]] | + | [[Category:Team:Paris-Saclay/Project Gemote|5]] |
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<div class="st-content"> | <div class="st-content"> | ||
- | + | <p><b><i>6th august</i></b></p> | |
- | + | <ol> | |
+ | <li>Sending of the B1 sample to sequencing with two pairs of primers</li> | ||
+ | <ol> | ||
+ | <li>K-274100 Forward and Reverse</li> | ||
+ | <li>Plasmid pSB1A2 Forward and Reverse</li> | ||
+ | </ol> | ||
+ | </ol></br> | ||
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+ | <p><b><i>7th august</i></b></p> | ||
+ | <ol> | ||
+ | <li>Liquid culture of B1 in order to prepare a glycerol stock</li> | ||
+ | <li>Receipt of new primers</li> | ||
+ | <ol> | ||
+ | <li>2 Forward</li> | ||
+ | <li>3 Reverse</li> | ||
+ | <li>Plasmid Reverse</li> | ||
+ | </ol> | ||
+ | </ol></br> | ||
+ | <p><b><i>8th august</i></b></p> | ||
+ | <p>Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid. | ||
+ | <img width="350px" src="https://static.igem.org/mediawiki/2012/c/c3/Week10-2.jpg"/> | ||
+ | <img width="500px" src="https://static.igem.org/mediawiki/2012/8/8b/Week10-3.jpg"/> | ||
+ | </p></br> | ||
+ | <p><b><i>9th august</i></b></p> | ||
+ | <p>New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before. | ||
+ | <img width="350px" src="https://2012.igem.org/File:Week10-4.jpg"/> | ||
+ | Digestion by EcoRI of the plasmid that contains BBa_K274100. | ||
+ | </p></br> | ||
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- | <a href="#"> | + | <a href="#">Eleventh Week<span class="st-arrow">Open or Close</span></a> |
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{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} |
Latest revision as of 06:46, 26 September 2012
GEMOTE
GEMOTE
-
First WeekOpen or Close
-
Second WeekOpen or Close
-
Third WeekOpen or Close
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Fourth WeekOpen or Close
-
Fifth WeekOpen or Close
-
Sixth WeekOpen or Close
-
Seventh WeekOpen or Close
-
Eighth WeekOpen or Close
-
Ninth WeekOpen or Close
-
Tenth WeekOpen or Close
6th august
- Sending of the B1 sample to sequencing with two pairs of primers
- K-274100 Forward and Reverse
- Plasmid pSB1A2 Forward and Reverse
7th august
- Liquid culture of B1 in order to prepare a glycerol stock
- Receipt of new primers
- 2 Forward
- 3 Reverse
- Plasmid Reverse
8th august
Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.
9th august
New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before. Digestion by EcoRI of the plasmid that contains BBa_K274100.
-
Eleventh WeekOpen or Close
-
Thirteenth WeekOpen or Close
Follow us !