Team:BAU-Indonesia/ProjectDesc

From 2012.igem.org

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<h2>Safety</h2>
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<h2>Project Description == Plastic Terminator == </h2><br> <h2>"The plastics destroy us, or we destroy them"</h2> <br><br>
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    <strong>1. Would any of your project ideas raise safety issues in
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    <strong>Goal:
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    terms of:
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                                         </strong>
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  </h3><br>                                     
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  </h3>
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  <h4><strong>Researcher Safety</strong><br></h4>
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    <h4>the goal of this project is to design a polyethylene-biodegradator enzyme which is could
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    <h4>We have isolated and cultured the bacteria sample from landfill
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be applicated to biodegradable and non-biodegradable polyethylene (PE).</h4><br>  
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    at Galuga, West Java Indonesia. To prevent contamination by some
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    harmfull bacteria, we have followed the instruction of safety
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    procedure, such as using sensigloves, mask, and washing hand
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    after sampling. Hazardous chemicals that we used are aclrilamide
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    and ethidium bromyde. Acrylamide (or acrylic amide) is a simple
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    organic compound with the chemical formula C3H5NO. It's
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    potentially hazardous to health (cancer or carcinogenic).
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    Acrylamide can form long polymer chains known as polyacrylamide,
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    which is also carcinogenic. Inhalation: irritation,
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    disorientation, burning, convulsions. Contact with skin:
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    irritation, disorientation. Eye contact: irritation, a burning
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    sensation of the eyes, eye damage.
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    Ethidium bromide will reduce the rate of migration of linear DNA
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    molecules by 15%. This solution is extremely dangerous and  
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    carcinogenic. All solutions which contained ethidium bromide
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    should be decontaminated before disposal. Ethidium Bromide
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    (EtBr) will fluoresce under UV exposure. These dyes can be added
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    to the gel and buffer so there is no staining after
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    electrophoresis process. The advantage is more practical, but
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    the possibility of EtBr contamination is greater. Due to the
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    carcinogenic factor, it must be avoided for direct contact.<br> </h4>
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    <h4><strong>Public Safety</strong><br></h4>
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        <h4><strong>Why is it an interesting problem?</strong><br></h4>
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    <h4>We have ethics to minimize the danger possibility to public. We
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    <h4>There is no enzyme which is capable as a biological agent for degrading plastics and can work without pre-treatment. </h4>
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    usually wear single-used gloves, covered shoes, lab coat, and
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    mask so the accumulation of materials and impurities are not
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    exposed to participant directly. We have a liquid discharge
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    valves which equipped with running water, so it won't settle. We
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    also have a separated solid waste disposal for potentially
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    hazardous materials. Those ethics are rules in our work on this
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    project. </h4>
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    <h4><strong>Environmental Safety</strong><br></h4>
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        <h4><strong>Our design project:</strong><br></h4>
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  <h4> No harmful effect on our enzyme product. E. coli bacteria which
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  <h4> During the process of degradation, PE plastic should be given a physical treatment, including UV rays and prooxidant so bacteria allows to degrade it easily. We will use landfill plastic and there is an expectation for breaking the hydrophobic bonds into hydrophilic bonds on the surface of plastic indirectly, then the PE plastic is easy to be degraded. The target is an engineered-biodegradable plastic enzymes.
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    inserted by  Cutinase gene has been set for its life period by
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Finally, We hope that it will be useful economically for landfill plastic industries, especially for some developing countries ; furthermore, it can be applied as an enzyme deployment system directly in an open junk space.
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    sonicator so there'll no blooming act in nature. Destruction of  
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    PET by using our products is done at indoor laboratory so the
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    waste does not impact to the environment directly. The enzyme
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    does not cause further effect especially for chemical / physical
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    danger.No harmful effect on our enzyme product. E. coli bacteria
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    which inserted by  Cutinase gene has been set for its life
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    period by sonicator so there'll no blooming act in nature.
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    Destruction of PET by using our products is done at indoor
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    laboratory so the waste does not impact to the environment
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    directly. The enzyme does not cause further effect especially
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      for chemical / physical danger.
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  </h4>                                      <br>
  </h4>                                      <br>
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  <h3> <strong>2. Do any of the new BioBrick parts (or devices) that you
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    made this year raise safety issues? If yes, Did you document these issues in the Registry?</strong> </h3><br>
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    <h4>No, we didn't document the safety issue to the registry, but our
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    procedure was according the Standard Operational Procedure of
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    Molecular Biology Laboratory and Microbiology laboratory in IPB
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    (Bogor Agricultural University). So we think our project hasn't
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    any safety risk during we follow the procedure carefully.</h4><br>
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    <h4><strong>How did you manage to handle the safety issue?</strong></h4><br>
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  <h4> To handle the security issue of our work, we always consult with
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    the team advisor and instructor who handle the laboratory
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    security work in IPB. Our security is guaranteed by following
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    the standard operating procedures which provided in each
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    laboratory. As a mechanism to ensure the safety of our work,
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    first of all we started it by product testing step in the
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    laboratory before applying it in nature, so when the result is
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    guaranteed safely, it can be applied in nature.</h4><br>
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    <h4><strong>How could other teams learn from your experience?</strong></h4><br>
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  <h4> Other teams can learn from the procedure that we have used. We
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    use the common procedure that is used by molecular researchers
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    at the laboratory, so its safety has been tested (as long as we
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      follow the standard operation procedure had been given).                                     
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      </h4><br>
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    <h3><strong>3. Is there a local biosafety group, committee, or review
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    board at your institution? </strong><h3> <br>
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      <h4><strong>Which specific biosafety rules or guidelines do you have to
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    consider in your country?</strong></h4><br>
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  <h4> We have no local biosafety committee at our institution but
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    there are Biosafety rules or guidelines in our country. Please
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    see below :<br>
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    1. Regulation Law No. 32 of 2009 about Environmental
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    Protection & Management<br>
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    2. Law No. 7 of 1996 on Food<br>
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    3. Law No. 18 of 2002 on National System
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    Research,Development and Application of Science and
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    Technology<br>
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    4. Law No. 18 of 2004 on Plantation<br>
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    5. Law No. 23 of 1992 on Health<br>
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    6. Law No. 23 of 1992 on Animal, Fish, and Plant
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    Quarantine<br>
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    7. Law No. 41 of 1999 on Forestry<br>
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    8. Government Regulation No. 21 of 2005 on Biosafety of
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    Genetically Engineered Product<br>
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    9. Presidential Decree 39 of 2010 on Biosafety Commision of
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    Genetically Engineered Product.<br>
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    10. INSTITUTIONAL ARRANGEMENT Biosafety Commision
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    Established by Presidential Decree No. 39/2010 <br>
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    11. Biosafety Technical Team In the establishment
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    process.<br>
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    12. Biosafety Clearing House Regarding Article 20 of the
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    Cartagena Protocol, Indonesia has been establish a Biosafety
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    Clearing House to facilitate the exchange of scientific,
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    technical , environmental and legalinformation on LMO. BCH is
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    coordinated by Ministry of Environment in cooperation with
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    Indonesia Institute of Science Secretariat of Biosafety
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    Commission.<br>
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    13. Law 32/2009. Every activities may create important risk
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    on environment, biodiversity and human healthand human safety
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    have to carry out Environmental Risk Analysis, Risk Assessment,
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      Risk Management, Risk Communication.                                                                                                                   
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      </h4><br>
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        <h3><strong>4. Do you have any other ideas how to deal with safety
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        issues that could be useful for future iGEM competitions? How
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        could parts, devices and systems be made even safer through
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          biosafety engineering? </strong>
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      </h3>                                      <br>
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      <h4>To ensure the biosafety, it will be better to give more
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        certification letter about the safety of materials which
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        provided to the participants. Each institution must have their
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        own fire fighters and laboratory clinic centre.
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        To minimize the hazardous chemicals we can use the synthetic
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        biology engineered system. The equipment which is used is fairly
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        easy to control for these biosafety ethics. The example of
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        recommended system that has better security is JSA (Job Safety
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        Analyst).</h4>
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Revision as of 05:12, 26 September 2012


Metamorphosis Design Free Css Templates

Project Description == Plastic Terminator ==


"The plastics destroy us, or we destroy them"



Goal:

the goal of this project is to design a polyethylene-biodegradator enzyme which is could be applicated to biodegradable and non-biodegradable polyethylene (PE).


Why is it an interesting problem?

There is no enzyme which is capable as a biological agent for degrading plastics and can work without pre-treatment.


Our design project:

During the process of degradation, PE plastic should be given a physical treatment, including UV rays and prooxidant so bacteria allows to degrade it easily. We will use landfill plastic and there is an expectation for breaking the hydrophobic bonds into hydrophilic bonds on the surface of plastic indirectly, then the PE plastic is easy to be degraded. The target is an engineered-biodegradable plastic enzymes. Finally, We hope that it will be useful economically for landfill plastic industries, especially for some developing countries ; furthermore, it can be applied as an enzyme deployment system directly in an open junk space.


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