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Team:UT-Tokyo/Notebook
From 2012.igem.org
(Difference between revisions)
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<p></html> | <p></html> | ||
==== May 29th ==== | ==== May 29th ==== | ||
| - | * | + | *Making LB medium |
==== May 30th ==== | ==== May 30th ==== | ||
| - | * | + | *Making LB Agar |
| - | * | + | *Making LB Amp Plates |
==== May 31st ==== | ==== May 31st ==== | ||
| - | * | + | *Preculture |
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==== June 5th ==== | ==== June 5th ==== | ||
| - | * | + | *Plating JM109 |
==== June 6th ==== | ==== June 6th ==== | ||
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==== June 7th ==== | ==== June 7th ==== | ||
| - | * | + | *Measuring OD by spectroscope |
| - | * | + | *Meking electrocompetent cells |
| - | * | + | *Electroporating of a test plasmid |
==== June 8th ==== | ==== June 8th ==== | ||
| - | * | + | *Caluculatiing transformation efficiency |
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<div id="Week03" class="Weeks"> | <div id="Week03" class="Weeks"> | ||
<p></html> | <p></html> | ||
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<html></p> | <html></p> | ||
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==== June 26th ==== | ==== June 26th ==== | ||
| - | * | + | *Making LB tetracycline plate |
==== June 27th ==== | ==== June 27th ==== | ||
==== June 28th ==== | ==== June 28th ==== | ||
| - | * | + | *Making LB kanamycin plate and chloramphenicol plate |
| - | * | + | *Transformation with a test plasmid |
==== June 29th ==== | ==== June 29th ==== | ||
| - | * | + | *Transformation with #1(constitutive promoter) and #11(RBS) |
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<h3 onclick="ShowNote('Week06');" onkeypress="ShowNote('Week06');">6th week: 7/1-7/7</h3> | <h3 onclick="ShowNote('Week06');" onkeypress="ShowNote('Week06');">6th week: 7/1-7/7</h3> | ||
<div id="Week06" class="Weeks"> | <div id="Week06" class="Weeks"> | ||
| + | ==== July 3rd==== | ||
| + | *Making K solution | ||
| + | *Colony PCR DNA fragment assumed as fhlA (#6) for cloning | ||
| + | |||
| + | ==== July 4th ==== | ||
| + | *Miniprep #1, #11 | ||
| + | |||
| + | ==== July 5th ==== | ||
| + | *Making 1% agarose solution | ||
| + | *Electrophoresis (#6) | ||
| + | *Digest (#1_EP, #11-_EP) | ||
| + | |||
| + | ==== July 6th ==== | ||
| + | *Colony PCR (#6) | ||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | <h3 onclick="ShowNote('Week07');" onkeypress="ShowNote('Week07');">7th week: 7/8-7/14</h3> | ||
| + | <div id="Week07" class="Weeks"> | ||
| + | ==== July 9th ==== | ||
| + | *Electrophoresis (#6) | ||
| + | |||
| + | ==== July 10th ==== | ||
| + | *Transformation | ||
| + | **#3(RBS medium), #5(d.term), #20(pLac) | ||
| + | |||
| + | ==== July 11th ==== | ||
| + | *Preculture | ||
| + | ** #3, #5, #20 | ||
| + | *Gel Extraction(#6) | ||
| + | |||
| + | ==== July 12th ==== | ||
| + | *The continuation of Gel Extraction(#6) | ||
| + | *Miniprep | ||
| + | ** #3, #5, #20 | ||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | <h3 onclick="ShowNote('Week08');" onkeypress="ShowNote('Week08');">8th week: 7/15-7/21</h3> | ||
| + | <div id="Week08" class="Weeks"> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | <h3 onclick="ShowNote('Week09');" onkeypress="ShowNote('Week09');">9th week: 7/22-7/28</h3> | ||
| + | <div id="Week09" class="Weeks"> | ||
</div> | </div> | ||
Revision as of 04:25, 26 September 2012
Notebook
このページの概要を、簡単に記述。
May
1st week: 5/27-6/2
May 29th
- Making LB medium
May 30th
- Making LB Agar
- Making LB Amp Plates
May 31st
- Preculture
June
2nd week: 6/3-6/9
June 5th
- Plating JM109
June 6th
- preculture
June 7th
- Measuring OD by spectroscope
- Meking electrocompetent cells
- Electroporating of a test plasmid
June 8th
- Caluculatiing transformation efficiency
3rd week: 6/10-6/16
4th week: 6/17-6/23
5th week: 6/24-6/30
==== June 26th ====
*Making LB tetracycline plate
==== June 27th ====
==== June 28th ====
*Making LB kanamycin plate and chloramphenicol plate
*Transformation with a test plasmid
==== June 29th ====
*Transformation with #1(constitutive promoter) and #11(RBS)
2012/07
6th week: 7/1-7/7
==== July 3rd====
*Making K solution
*Colony PCR DNA fragment assumed as fhlA (#6) for cloning
==== July 4th ====
*Miniprep #1, #11
==== July 5th ====
*Making 1% agarose solution
*Electrophoresis (#6)
*Digest (#1_EP, #11-_EP)
==== July 6th ====
*Colony PCR (#6)
7th week: 7/8-7/14
==== July 9th ====
*Electrophoresis (#6)
==== July 10th ====
*Transformation
**#3(RBS medium), #5(d.term), #20(pLac)
==== July 11th ====
*Preculture
** #3, #5, #20
*Gel Extraction(#6)
==== July 12th ====
*The continuation of Gel Extraction(#6)
*Miniprep
** #3, #5, #20
8th week: 7/15-7/21
9th week: 7/22-7/28
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
