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Team:Macquarie Australia/Protocols/RD
From 2012.igem.org
(Difference between revisions)
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<html> | <html> | ||
<center><h1>Restriction Digest</h1></center> | <center><h1>Restriction Digest</h1></center> | ||
| - | <p></p> | + | <p>We prepared Master Mixes containing to following volumes,</p> |
| + | <center><table><tr><td><table border="3" cellpadding="4" cellspacing="0"> | ||
| + | <tr><td colspan="2">EcoR1 + Spe1 Master Mix</td></tr> | ||
| + | <tr><td>Substrate</td><td>Volume (µL)</td></tr> | ||
| + | <tr><td>10x Buffer</td><td>20</td></tr> | ||
| + | <tr><td>EcoR1</td><td>2.5</td></tr> | ||
| + | <tr><td>Spe1</td><td>2.5</td></tr> | ||
| + | <tr><td>Water</td><td>75</td></tr> | ||
| + | <tr><td>Total</td><td>100</td></tr> | ||
| + | </table></td> | ||
| + | <td><table border="3" cellpadding="4" cellspacing="0"> | ||
| + | <tr><td colspan="2">EcoR1 + Spe1 Master Mix</td></tr> | ||
| + | <tr><td>Substrate</td><td>Volume (µL)</td></tr> | ||
| + | <tr><td>10x Buffer</td><td>20</td></tr> | ||
| + | <tr><td>EcoR1</td><td>2.5</td></tr> | ||
| + | <tr><td>Spe1</td><td>2.5</td></tr> | ||
| + | <tr><td>Water</td><td>75</td></tr> | ||
| + | <tr><td>Total</td><td>100</td></tr> | ||
| + | </table></td> | ||
| + | <td><table border="3" cellpadding="4" cellspacing="0"> | ||
| + | <tr><td colspan="2">EcoR1 + Spe1 Master Mix</td></tr> | ||
| + | <tr><td>Substrate</td><td>Volume (µL)</td></tr> | ||
| + | <tr><td>10x Buffer</td><td>20</td></tr> | ||
| + | <tr><td>EcoR1</td><td>2.5</td></tr> | ||
| + | <tr><td>Spe1</td><td>2.5</td></tr> | ||
| + | <tr><td>Water</td><td>75</td></tr> | ||
| + | <tr><td>Total</td><td>100</td></tr> | ||
| + | </table></td> | ||
| + | <td><table border="3" cellpadding="4" cellspacing="0"> | ||
| + | <tr><td colspan="2">EcoR1 + Spe1 Master Mix</td></tr> | ||
| + | <tr><td>Substrate</td><td>Volume (µL)</td></tr> | ||
| + | <tr><td>10x Buffer</td><td>20</td></tr> | ||
| + | <tr><td>EcoR1</td><td>2.5</td></tr> | ||
| + | <tr><td>Spe1</td><td>2.5</td></tr> | ||
| + | <tr><td>Water</td><td>75</td></tr> | ||
| + | <tr><td>Total</td><td>100</td></tr> | ||
| + | </table></td></table></center> | ||
</html> | </html> | ||
Revision as of 01:19, 26 September 2012
Restriction Digest
We prepared Master Mixes containing to following volumes,
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