Team:Exeter/lab book/3gip/wk6

From 2012.igem.org

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     <p><b><u>Monday 13th August</u></b></p>
     <p><b><u>Monday 13th August</u></b></p>
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<p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#1d1d1b"><u>Operon Constructipn 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p>
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<p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#57B947"><u>Operon Construction 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p>
<p></p><br>
<p></p><br>
<p><b><u>Tuesday 14th August</u></b></p>
<p><b><u>Tuesday 14th August</u></b></p>
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<p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#1d1d1b"><u>Operon Constructipn 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p>
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<p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#57B947"><u>Operon Construction 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p>
<p></p><br>
<p></p><br>
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<p>Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed. </p>
<p>Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed. </p>
<p>After the neutralisation solution had been added, the eppendorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. </p>
<p>After the neutralisation solution had been added, the eppendorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. </p>
-
<p>40ul of water was added instead of 40ul in order to make sure the concentration of DNA increased. </p>
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<p>40ul of water was added instead of 50ul in order to make sure the concentration of DNA increased. </p>
<p>All were nanodropped and only <i>wfcA</i> + BBa_B0014 and <i>wbnJ</i> + BBa_B0014 gave good concentrations. </p><br>
<p>All were nanodropped and only <i>wfcA</i> + BBa_B0014 and <i>wbnJ</i> + BBa_B0014 gave good concentrations. </p><br>
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<p><b><u>Wednesday 15th August –Friday 17th August</u></b></p>
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<p><b><u>Wednesday 15th August – Friday 17th August</u></b></p>
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<p>Preparation of presentation so no lab work. </p>  
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<p>Preparation for presentation so no lab work. </p>  
      
      
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Revision as of 22:52, 25 September 2012

ExiGEM2012 Lab Book 3GP wk6

The 3-Gene Inducible Plasmid: 13th - 17th August 2012

Monday 13th August

See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005)


Tuesday 14th August

See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005)


Wednesday 15th August

Morning

•Miniprepping

BBa_J13002 + wbnK BBa_B0014 in pSB1T3

wfcA + BBa_B0014 in pSB1T3

wbnJ + BBa_B0014 in pSB1T3

BBa_B0034 wbbC + BBa_B0014 in pSB1T3

Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed.

After the neutralisation solution had been added, the eppendorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation.

40ul of water was added instead of 50ul in order to make sure the concentration of DNA increased.

All were nanodropped and only wfcA + BBa_B0014 and wbnJ + BBa_B0014 gave good concentrations.


Wednesday 15th August – Friday 17th August

Preparation for presentation so no lab work.

Retrieved from "http://2012.igem.org/Team:Exeter/lab_book/3gip/wk6"