Team:LMU-Munich/Test

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{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo1.jpg}}
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==Our Team==
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{| style="color:black;" cellpadding="10" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
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{| style="color:black;" cellpadding="3" width="100%" cellspacing="0" border="0" align="center" style="text-align:left;"
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| style="width: 70%;background-color:#116f3d;" |
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| style="width: 100%;background-color: #EBFCE4;" |
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{|
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{| style="width: 100%" align="center"
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|[[File:Imamura, 2011 & McKenney, 2010.png|Protein distribution in spore coat of ''Bacillus subtilis''|610px]]
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|[[Image:IGEM2012_LMU_team_Korinna.jpg|Korinna|150px|center]]
 +
|[[Image:IGEM2012_LMU_team_Franzi.jpg|Franzi|150px]]
 +
|[[Image:IGEM2012_LMU_team_Tamara.jpg|Tamara|150px]]
 +
|[[Image:IGEM2012_LMU_team_Simon.jpg|Simon|150px]]
 +
|-
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| style="background-color:#EBFCE4;text-align:center;" |
 +
Korinna
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| style="background-color:#EBFCE4;text-align:center;" |
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Franzi
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| style="background-color:#EBFCE4;text-align:center;" |
 +
Tamara
 +
| style="background-color:#EBFCE4;text-align:center;" |
 +
Simon
|}
|}
|-
|-
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| style="width: 70%;background-color:#116f3d;" |
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| style="width: 100%;background-color: #EBFCE4;" |
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<font color="#EBFCE4">Protein distribution in spore coat of ''Bacillus subtilis''</font>
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{| style="width: 100%" align="center"
 +
|[[Image:IGEM2012_LMU_team_Julia.jpg|150px|center]]
 +
|[[Image:IGEM2012_LMU_team_Jenny.jpg|150px]]
 +
|[[Image:IGEM2012_LMU_team_Jara.jpg|150px]]
 +
|[[Image:IGEM2012_LMU_team_Thorsten.jpg|150px]]
|-
|-
 +
| style="background-color:#EBFCE4;text-align:center;" |
 +
Julia
 +
| style="background-color:#EBFCE4;text-align:center;" |
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Jenny
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| style="background-color:#EBFCE4;text-align:center;" |
 +
Jara
 +
| style="background-color:#EBFCE4;text-align:center;" |
 +
Thorsten
 +
|}
|}
|}
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{|
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| style="width: 70%;background-color: #EBFCE4;" |
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{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
 +
|style="width: 70%;background-color: #EBFCE4;" |
 +
<font color="#000000"; size="2">Korinna</font>
 +
|}
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| style="width: 70%;background-color: #EBFCE4;" |
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{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
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|style="width: 70%;background-color: #EBFCE4;" |
 +
<font color="#000000"; size="2">Franzi</font>
 +
|}
|-
|-
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| style="width: 30%;background-color:#116f3d;" |
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|}
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; Defintion and planning (12 weeks)
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|}
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| style="width: 40%;background-color:#ffffcc;text-align:left;" |
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-
;
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Image:IGEM2012_LMU_team_Korinna.jpg|Korinna
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*Definition of project/s
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Image:IGEM2012_LMU_team_Franzi.jpg|Franzi
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*Arrangement of work groups
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Image:IGEM2012_LMU_team_Tamara.jpg|Tamara
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*Definition of objectives
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Image:IGEM2012_LMU_team_Simon.jpg|Simon
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*Clarification of resources
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Image:IGEM2012_LMU_team_Julia.jpg|Julia
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*Identification of milestones
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Image:IGEM2012_LMU_team_Jenny.jpg|Jenny
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|-
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Image:IGEM2012_LMU_team_Jara.jpg|Jara
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| style="width: 30%;background-color:#FFEC8B;" |
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Image:IGEM2012_LMU_team_Thorsten.jpg|Prof. Dr. Mascher
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; Project Work (36 weeks)
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| style="width: 40%;background-color:#ffffcc;text-align:left;" |
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|[[Image:IGEM2012_LMU_team_Julia.jpg|Julia|100px]]
-
;
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|[[Image:IGEM2012_LMU_team_Jenny.jpg|Jenny|100px]]
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*Work group meetings
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|[[Image:IGEM2012_LMU_team_Jara.jpg|Jara|100px]]
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*Meetings with teacher and external partner
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|[[Image:IGEM2012_LMU_team_Thorsten.jpg|Prof. Dr. Mascher|100px]]
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*Independent team work
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-
*Presentation of intermediary results
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|-
|-
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| style="width: 30%;background-color:#FFEC8B;" |
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| style="width: 70%;background-color: #EBFCE4;" |
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; Presentation and evaluation (12 weeks)
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{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
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| style="width: 40%;background-color:#ffffcc;text-align:left;" |
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|style="width: 70%;background-color: #EBFCE4;" |
-
;  
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<font color="#000000"; size="2">''lacZ''-activity in Miller units in pSB<sub>Bs</sub>0K-P<sub>''spac''</sub> depending on ''B. subtilis'' growth phase.</font>
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*Presentation of results for school or external audience
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|}
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*Creation and discussion of individual seminar portfolios
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|}
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*Postprocessing (achievement of objectives, analysis of project work) 
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|}
|}
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We will create a toolbox of <i>Bacillus</i> BioBricks to contribute to the registry.
 
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This ''<b>Bacillus''B</b>io<b>B</b>rick<b>B</b>ox contains ''Bacillus'' specific:
 
 +
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
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| style="width: 70%;background-color: #EBFCE4;" |
 +
{|
 +
|[[File:LMU-Munich-0K-OD.png|500px|center]]
 +
|-
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| style="width: 70%;background-color: #EBFCE4;" |
 +
{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
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|style="width: 70%;background-color: #EBFCE4;" |
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<font color="#000000"; size="2">''lacZ''-activity in Miller units in pSB<sub>Bs</sub>0K-P<sub>''spac''</sub> depending on ''B. subtilis'' growth phase.</font>
 +
|}
 +
|}
 +
|}
 +
We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
 +
<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
 +
Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
 +
More details to come soon.
-
{| style="background-color:white" cellpadding="0" width="100%" cellspacing="0" border="0" align="center" style="text-align:center;"
 
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| style="width:20%"|
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<div class="box">
-
'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
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====Project Navigation====
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| style="width:20%"|
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{| width="100%" align="center" style="text-align:center;"
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
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|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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| style="width:20%"|
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|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
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|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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| style="width:20%"|
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|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Tags|Affinity tags]]
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|-
|-
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|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
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|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
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|[[File:LMU PromoterIconBC.png|100px]]
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|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
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|[[File:LMU Reporter.png|60px]]
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|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
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|[[File:Proteinaffinitytagbutton.png|50px]]
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|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
|}
|}
 +
</div>
 +
<div class="box">
 +
'''Project Navigation'''
 +
{| style= cellpadding="10" width="100%" cellspacing="1" align="center" style="text-align:center;"
 +
|style="background-color:white"|
 +
{|
 +
|style= "background-color:white"|
 +
[[File:Bacilluss_Intro.png|120px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction]]
 +
|style= "background-color:white"|
 +
[[Team:LMU-Munich/Bacillus_Introduction|''B. subtilis'' Intro]]
 +
|-
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|style=  "background-color:white"|
 +
[[File:BacillusBioBrickBox.png|120px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
 +
|style= "background-color:white"|
 +
[[Team:LMU-Munich/Bacillus_BioBricks|'''''Bacillus''B'''io'''B'''rick'''B'''ox]]
 +
|-
 +
|}
 +
|style="background-color:white"|
 +
{|
 +
|style="background-color:white"|
 +
[[File:SporeCoat.png|100px|link=https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins]]
 +
|style="background-color:white"|
 +
[[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]]
 +
|-
 +
|style="background-color:white"|
 +
[[File:GerminationSTOP.png|90px|link=https://2012.igem.org/Team:LMU-Munich/Germination_Stop]]
 +
|style="background-color:white"|
 +
[[Team:LMU-Munich/Germination_Stop|'''Germination'''STOP]]
 +
|}
 +
|}
 +
</div>
 +
 +
 +
{|
 +
| <html><a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction">
 +
<img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=50"/></a></html>
 +
| [[Team:LMU-Munich/Bacillus_Introduction|Intro to ''Bacillus subtilis'']]
-
{| class="colored"
 
-
|-
 
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!Name BioBrick
 
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!''E. coli'' res.
 
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!''B. subt.'' res.
 
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!Insertion locus
 
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!Description
 
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!Derived from
 
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!Reference
 
|-
|-
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!pSB<sub>Bs</sub>1C-<i>lacZ</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823021 <font color="#EBFCE4" size="2">(BBa_K823021) </font>]
+
 
-
|Amp
+
| <html><a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks">
-
|Cam
+
<img src="https://static.igem.org/mediawiki/2012/7/78/BacillusBioBrickBox.png" height=50"/></a></html>
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|amyE
+
 
-
|with <i>lacZ</i> reporter gene
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|[[Team:LMU-Munich/Bacillus_BioBricks|''Bacillus'' BioBrick'''BOX''']]
-
|pAC6
+
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke ''et al''.]
+
|-
|-
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!pSB<sub>Bs</sub>4S [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823022 <font color="#EBFCE4" size="2">(BBa_K823022)</font>]
+
 
-
|Amp
+
|<html><a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins">
-
|Spec
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<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=50"/></a></html>
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|thrC
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|empty
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|[[Team:LMU-Munich/Spore_Coat_Proteins|Sporo'''beads''']]
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|pDG1731
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|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
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|-
|-
-
!pSB<sub>Bs</sub>1C [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823023 <font color="#EBFCE4" size="2">(BBa_K823023)</font>]
+
 
-
|Amp
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|<html><a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop">
-
|Cam
+
<img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=50"/></a></html>
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|amyE
+
 
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|empty
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|[[Team:LMU-Munich/Germination_Stop|Germination'''STOP''']]
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|pDG1662
+
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|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
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|-
|-
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!pSB<sub>Bs</sub>4S-P<sub><i>Xyl</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823024 <font color="#EBFCE4" size="2">(BBa_K823024)</font>]
+
|}
-
|Amp
+
 
-
|Spec
+
 
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|thrC
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The promoters we submit are:
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|with Xylose-inducible promoter
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|pXT
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Pveg, PliaI, PliaG, plepA, Pxyl, Pxyl+XylR
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|[http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré ''et al.'']
+
 
 +
<p align="justify">We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
 +
 
 +
<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
 +
 
 +
Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
 +
 
 +
More details to come soon.
 +
 
 +
{| width="100%"  
 +
|valign="top" style="text-align:center;"|<font size="2">
 +
pSB<sub>Bs</sub>1C
 +
 
 +
pSB<sub>Bs</sub>4S
 +
 
 +
pSB<sub>Bs</sub>2E
 +
 
 +
pSB<sub>Bs</sub>1C-''lac''Z
 +
 
 +
pSB<sub>Bs</sub>3C-''lux''ABCDE
 +
 
 +
pSB<sub>Bs</sub>4S-P<sub>''Xyl''</sub>
 +
 
 +
pSB<sub>Bs</sub>0K-P<sub>''spac''</sub>
 +
</font>
 +
|valign="top"|<font size="2">
 +
Pveg
 +
 
 +
PliaI
 +
 
 +
PlepA
 +
 
 +
Anderson
 +
 
 +
</font>
 +
|valign="top"|<font size="2">
 +
mkate2
 +
 
 +
luc
 +
 
 +
lacZ
 +
 
 +
</font>
 +
|valign="top"|<font size="2">
 +
Flag
 +
 
 +
His
 +
 
 +
cMyc
 +
 
 +
Strep
 +
 
 +
HA
 +
</font>
|-
|-
-
!pSB<sub>Bs</sub>3C-<i>luxABCDE</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 <font color="#EBFCE4" size="2">(BBa_K823025)</font>]
+
|}
-
|Amp
+
 
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|Cam
+
 
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|sacA
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<div class="box" style= "background-color:#e4f1d7">
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|with <i>luxABCDE</i> reporter cassette
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==='''''Bacillus''B'''io'''B'''rick'''B'''ox===
-
|pAH328
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</div>
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|[http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch ''et al''.]
+
 
 +
<div class="box">
 +
====Anderson Promoter Evaluation====
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">Evaluation of the Anderson library in ''B. subtilis'' with pSB<sub>Bs</sub>3C-''lux'' (luminescence) and pSB<sub>Bs</sub>1C-''lacZ''
 +
[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Anderson]]</p>
 +
|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
 +
|}
 +
</div>
 +
 
 +
<div class="box">
 +
====Anderson Promoter Evaluation====
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">Evaluation of the Anderson library in ''B. subtilis'' with pSB<sub>Bs</sub>3C-''lux'' (luminescence) and pSB<sub>Bs</sub>1C-''lacZ''</p>
 +
|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
|-
|-
-
!pSB<sub>Bs</sub>0K-P<sub><i>spac</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 <font color="#EBFCE4" size="2">(BBa_K823026)</font>]
+
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Anderson]]
-
|Amp
+
|}
-
|Kan
+
</div>
-
|replicative
+
 
-
|with IPTG inducible promoter
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<div class="box">
-
|pDG148
+
{| width="100%"
-
|[http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph ''et al.'']
+
 
 +
| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
 +
| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
 +
| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
 +
| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags|Affinity tags]]
|-
|-
-
!pSB<sub>Bs</sub>2E [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823027 <font color="#EBFCE4" size="2">(BBa_K823027)</font>]
+
|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
-
|Amp
+
|[[File:LMU PromoterIconBC.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters]]
-
|MLS
+
|[[File:LMU Reporter.png|60px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters]]
-
|lacA
+
|[[File:Proteinaffinitytagbutton.png|50px|link=Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags]]
-
|empty
+
-
|pAX01
+
-
|[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl ''et al.'']
+
|-
|-
|}
|}
 +
</div>
 +
 +
 +
 +
 +
 +
 +
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Latest revision as of 22:02, 25 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo1.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Test"