Team:LMU-Munich/Test

From 2012.igem.org

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Latest revision as of 22:02, 25 September 2012

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

[ more news ]

Our Team


Korinna	Franzi	Tamara	Simon


Julia	Jenny	Jara	Thorsten

| style="width: 70%;background-color: #EBFCE4;" |

Korinna

| style="width: 70%;background-color: #EBFCE4;" |

Franzi

|- |} |}

| | | | |- | style="width: 70%;background-color: #EBFCE4;" |

lacZ-activity in Miller units in pSB_Bs0K-P_spac depending on B. subtilis growth phase.

|} |}

lacZ-activity in Miller units in pSB_Bs0K-P_spac depending on B. subtilis growth phase.

We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in Bacillus subtilis with pSB_Bs3C-luxABCDE. The data will be online in our Data section soon.</p>

Furthermore we plan to submit reporter genes optimized for Bacillus subtilis, namely GFP, lacZ, luc and mKate2.

Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA). More details to come soon.

Project Navigation


Bacillus Intro	Bacillus BioBrickBox	Sporobeads	Germination STOP

Project Navigation

	B. subtilis Intro
	BacillusBioBrickBox

	Sporobeads
	GerminationSTOP

	Intro to Bacillus subtilis
	Bacillus BioBrickBOX
	Sporobeads
	GerminationSTOP

The promoters we submit are:

Pveg, PliaI, PliaG, plepA, Pxyl, Pxyl+XylR

Furthermore we plan to submit reporter genes optimized for Bacillus subtilis, namely GFP, lacZ, luc and mKate2.

Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).

More details to come soon.

pSB_Bs1C

pSB_Bs4S

pSB_Bs2E

pSB_Bs1C-lacZ

pSB_Bs3C-luxABCDE

pSB_Bs4S-P_Xyl

pSB_Bs0K-P_spac

Pveg

PliaI

PlepA

Anderson

mkate2

luc

lacZ

Flag

His

cMyc

Strep

BacillusBioBrickBox

Anderson Promoter Evaluation

Evaluation of the Anderson library in B. subtilis with pSB_Bs3C-lux (luminescence) and pSB_Bs1C-lacZ

Anderson Promoter Evaluation


Evaluation of the Anderson library in B. subtilis with pSB_Bs3C-lux (luminescence) and pSB_Bs1C-lacZ

Vectors	Promoters	Reporters	Affinity tags

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Test"

@@ Line 2: / Line 2: @@
 {{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo1.jpg}}
+==Our Team==
+{| style="color:black;" cellpadding="3" width="100%" cellspacing="0" border="0" align="center" style="text-align:left;"
+| style="width: 100%;background-color: #EBFCE4;" |
+{| style="width: 100%" align="center"
+|[[Image:IGEM2012_LMU_team_Korinna.jpg|Korinna|150px|center]]
+|[[Image:IGEM2012_LMU_team_Franzi.jpg|Franzi|150px]]
+|[[Image:IGEM2012_LMU_team_Tamara.jpg|Tamara|150px]]
+|[[Image:IGEM2012_LMU_team_Simon.jpg|Simon|150px]]
+|-
+| style="background-color:#EBFCE4;text-align:center;" |
+Korinna
+| style="background-color:#EBFCE4;text-align:center;" |
+Franzi
+| style="background-color:#EBFCE4;text-align:center;" |
+Tamara
+| style="background-color:#EBFCE4;text-align:center;" |
+Simon
+|}
+|-
+| style="width: 100%;background-color: #EBFCE4;" |
+{| style="width: 100%" align="center"
+|[[Image:IGEM2012_LMU_team_Julia.jpg|150px|center]]
+|[[Image:IGEM2012_LMU_team_Jenny.jpg|150px]]
+|[[Image:IGEM2012_LMU_team_Jara.jpg|150px]]
+|[[Image:IGEM2012_LMU_team_Thorsten.jpg|150px]]
+|-
+| style="background-color:#EBFCE4;text-align:center;" |
+Julia
+| style="background-color:#EBFCE4;text-align:center;" |
+Jenny
+| style="background-color:#EBFCE4;text-align:center;" |
+Jara
+| style="background-color:#EBFCE4;text-align:center;" |
+Thorsten
+|}
+|}
-{| class="colored"
+| style="width: 70%;background-color: #EBFCE4;" |
-|-
+{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
-!Name BioBrick
+|style="width: 70%;background-color: #EBFCE4;" |
-!''E. coli'' res.
+<font color="#000000"; size="2">Korinna</font>
-!''B. subt.'' res.
+|}
-!Insertion locus
+| style="width: 70%;background-color: #EBFCE4;" |
-!Description
+{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
-!Derived from
+|style="width: 70%;background-color: #EBFCE4;" |
-!Reference
+<font color="#000000"; size="2">Franzi</font>
+|}
 |-
-!pSB<sub>Bs</sub>1C-<i>lacZ</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823021 <font color="#EBFCE4" size="2">(BBa_K823021) </font>]
+|}
-|Amp
+|}
-|Cam
-|amyE
+Image:IGEM2012_LMU_team_Korinna.jpg|Korinna
-|with <i>lacZ</i> reporter gene
+Image:IGEM2012_LMU_team_Franzi.jpg|Franzi
-|pAC6
+Image:IGEM2012_LMU_team_Tamara.jpg|Tamara
-|[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke ''et al''.]
+Image:IGEM2012_LMU_team_Simon.jpg|Simon
+Image:IGEM2012_LMU_team_Julia.jpg|Julia
+Image:IGEM2012_LMU_team_Jenny.jpg|Jenny
+Image:IGEM2012_LMU_team_Jara.jpg|Jara
+Image:IGEM2012_LMU_team_Thorsten.jpg|Prof. Dr. Mascher
+|[[Image:IGEM2012_LMU_team_Julia.jpg|Julia|100px]]
+|[[Image:IGEM2012_LMU_team_Jenny.jpg|Jenny|100px]]
+|[[Image:IGEM2012_LMU_team_Jara.jpg|Jara|100px]]
+|[[Image:IGEM2012_LMU_team_Thorsten.jpg|Prof. Dr. Mascher|100px]]
 |-
-!pSB<sub>Bs</sub>4S [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823022 <font color="#EBFCE4" size="2">(BBa_K823022)</font>]
+| style="width: 70%;background-color: #EBFCE4;" |
-|Amp
+{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
-|Spec
+|style="width: 70%;background-color: #EBFCE4;" |
-|thrC
+<font color="#000000"; size="2">''lacZ''-activity in Miller units in pSB<sub>Bs</sub>0K-P<sub>''spac''</sub> depending on ''B. subtilis'' growth phase.</font>
-|empty
+|}
-|pDG1731
+|}
-|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
+|}
+{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
+| style="width: 70%;background-color: #EBFCE4;" |
+{|
+|[[File:LMU-Munich-0K-OD.png|500px|center]]
 |-
-!pSB<sub>Bs</sub>1C [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823023 <font color="#EBFCE4" size="2">(BBa_K823023)</font>]
+| style="width: 70%;background-color: #EBFCE4;" |
-|Amp
+{| style="color:black;" cellpadding="0" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
-|Cam
+|style="width: 70%;background-color: #EBFCE4;" |
-|amyE
+<font color="#000000"; size="2">''lacZ''-activity in Miller units in pSB<sub>Bs</sub>0K-P<sub>''spac''</sub> depending on ''B. subtilis'' growth phase.</font>
-|empty
+|}
-|pDG1662
+|}
-|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
+|}
+We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
+<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
+Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
+More details to come soon.
+<div class="box">
+====Project Navigation====
+{| width="100%" align="center" style="text-align:center;"
+|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
+|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
+|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
+|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
 |-
-!pSB<sub>Bs</sub>4S-P<sub><i>Xyl</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823024 <font color="#EBFCE4" size="2">(BBa_K823024)</font>]
+|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
-|Amp
+|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
-|Spec
+|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
-|thrC
+|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
-|with Xylose-inducible promoter
+|}
-|pXT
+</div>
-|[http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré ''et al.'']
+<div class="box">
+'''Project Navigation'''
+{| style= cellpadding="10" width="100%" cellspacing="1" align="center" style="text-align:center;"
+|style="background-color:white"|
+{|
+|style= "background-color:white"|
+[[File:Bacilluss_Intro.png|120px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction]]
+|style= "background-color:white"|
+[[Team:LMU-Munich/Bacillus_Introduction|''B. subtilis'' Intro]]
 |-
-!pSB<sub>Bs</sub>3C-<i>luxABCDE</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 <font color="#EBFCE4" size="2">(BBa_K823025)</font>]
+|style=  "background-color:white"|
-|Amp
+[[File:BacillusBioBrickBox.png|120px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
-|Cam
+|style= "background-color:white"|
-|sacA
+[[Team:LMU-Munich/Bacillus_BioBricks|'''''Bacillus''B'''io'''B'''rick'''B'''ox]]
-|with <i>luxABCDE</i> reporter cassette
-|pAH328
-|[http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch ''et al''.]
 |-
-!pSB<sub>Bs</sub>0K-P<sub><i>spac</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 <font color="#EBFCE4" size="2">(BBa_K823026)</font>]
+|}
-|Amp
+|style="background-color:white"|
-|Kan
+{|
-|replicative
+|style="background-color:white"|
-|with IPTG inducible promoter
+[[File:SporeCoat.png|100px|link=https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins]]
-|pDG148
+|style="background-color:white"|
-|[http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph ''et al.'']
+[[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]]
 |-
-!pSB<sub>Bs</sub>2E [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823027 <font color="#EBFCE4" size="2">(BBa_K823027)</font>]
+|style="background-color:white"|
-|Amp
+[[File:GerminationSTOP.png|90px|link=https://2012.igem.org/Team:LMU-Munich/Germination_Stop]]
-|MLS
+|style="background-color:white"|
-|lacA
+[[Team:LMU-Munich/Germination_Stop|'''Germination'''STOP]]
-|empty
+|}
-|pAX01
+|}
-|[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl ''et al.'']
+</div>
+{|
+| <html><a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction">
+<img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=50"/></a></html>
+| [[Team:LMU-Munich/Bacillus_Introduction|Intro to ''Bacillus subtilis'']]
+|-
+| <html><a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks">
+<img src="https://static.igem.org/mediawiki/2012/7/78/BacillusBioBrickBox.png" height=50"/></a></html>
+|[[Team:LMU-Munich/Bacillus_BioBricks|''Bacillus'' BioBrick'''BOX''']]
+|-
+|<html><a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins">
+<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=50"/></a></html>
+|[[Team:LMU-Munich/Spore_Coat_Proteins|Sporo'''beads''']]
+|-
+|<html><a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop">
+<img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=50"/></a></html>
+|[[Team:LMU-Munich/Germination_Stop|Germination'''STOP''']]
 |-
 |}
+The promoters we submit are:
+Pveg, PliaI, PliaG, plepA, Pxyl, Pxyl+XylR
+<p align="justify">We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
+<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
+Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
+More details to come soon.
+{| width="100%"
+|valign="top" style="text-align:center;"|<font size="2">
+pSB<sub>Bs</sub>1C
+pSB<sub>Bs</sub>4S
+pSB<sub>Bs</sub>2E
+pSB<sub>Bs</sub>1C-''lac''Z
+pSB<sub>Bs</sub>3C-''lux''ABCDE
+pSB<sub>Bs</sub>4S-P<sub>''Xyl''</sub>
+pSB<sub>Bs</sub>0K-P<sub>''spac''</sub>
+</font>
+|valign="top"|<font size="2">
+Pveg
+PliaI
+PlepA
+Anderson
+</font>
+|valign="top"|<font size="2">
+mkate2
+luc
+lacZ
+</font>
+|valign="top"|<font size="2">
+Flag
+His
+cMyc
+Strep
+HA
+</font>
+|-
+|}
+<div class="box" style= "background-color:#e4f1d7">
+==='''''Bacillus''B'''io'''B'''rick'''B'''ox===
+</div>
+<div class="box">
+====Anderson Promoter Evaluation====
+{| "width=100%" style="text-align:center;"|
+|<p align="justify">Evaluation of the Anderson library in ''B. subtilis'' with pSB<sub>Bs</sub>3C-''lux'' (luminescence) and pSB<sub>Bs</sub>1C-''lacZ''
+[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Anderson]]</p>
+|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
+|}
+</div>
+<div class="box">
+====Anderson Promoter Evaluation====
+{| "width=100%" style="text-align:center;"|
+|<p align="justify">Evaluation of the Anderson library in ''B. subtilis'' with pSB<sub>Bs</sub>3C-''lux'' (luminescence) and pSB<sub>Bs</sub>1C-''lacZ''</p>
+|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
+|-
+! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Anderson]]
+|}
+</div>
+<div class="box">
+{| width="100%"
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags|Affinity tags]]
+|-
+|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
+|[[File:LMU PromoterIconBC.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters]]
+|[[File:LMU Reporter.png|60px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters]]
+|[[File:Proteinaffinitytagbutton.png|50px|link=Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags]]
+|-
+|}
+</div>
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 {{:Team:LMU-Munich/Templates/Page Footer}}