Team:Bielefeld-Germany/Labjournal/week22

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Latest revision as of 21:33, 25 September 2012

Week 22 (09/24 - 09/30/12)

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Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

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 ==Week 22 (09/24 - 09/30/12)==
-===Monday September 24th===
-* '''Team Cultivation & Purification:'''[[File:Bielefeld2012_0924.jpg|300px|thumb|right|'''Figure 1: Cultivation from [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21#Sunday_September_23rd 09/23]''' of ''E. coli'' KRX with <partinfo>BBa_K729006</partinfo> , ''E. coli'' KRX without plasmid (negative control) and with <partinfo>BBa_K863005</partinfo> (positive control). 60&nbsp;mL in 250&nbsp;mL flasks without baffles, [https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium], 60&nbsp;µg/mL chloramphenicol, 37&nbsp;°C for 12&nbsp;hours.]]
-** For collaboration with [https://2012.igem.org/Team:University_College_London UCL London]: Made celldisruption via sonification and SDS-Page from lysates. The bands were analysed by MALDI-TOF.
-===Tuesday September 25th===
-===Wednesday September 26th===
-===Thursday September 27th===
-===Friday September 28st===
-===Saturday September 29nd===
-===Sunday September 30rd===
-Sunday
 {{Team:Bielefeld/Sponsoren}}