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Team:Trieste/data
From 2012.igem.org
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we have dimontrated that it is a very strong and strictly regulated promoter.Thanks to its rapid reactivity it's one of the first candidate for the expression and production of toxin in case of necessity. | we have dimontrated that it is a very strong and strictly regulated promoter.Thanks to its rapid reactivity it's one of the first candidate for the expression and production of toxin in case of necessity. | ||
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| - | - | + | -BBa_K875003, CymR: stringenza |
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we have succesfully expressed the CymR. We have also show his stringency as repressor of the T5 cumate operator that makes the all system a very useful mechanism for an high specificity expression. | we have succesfully expressed the CymR. We have also show his stringency as repressor of the T5 cumate operator that makes the all system a very useful mechanism for an high specificity expression. | ||
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| - | - | + | -BBa_K875004, LPP-OmpA-scFv: |
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we have verified the production of this protein at high yield. OmpA is a very powerful method for displaying proteins on the external membrane, in this case the antibody scFv 54.6. | we have verified the production of this protein at high yield. OmpA is a very powerful method for displaying proteins on the external membrane, in this case the antibody scFv 54.6. | ||
| + | <br/> | ||
| + | <br/> | ||
| + | The part we have improved: | ||
| + | <br/> | ||
| + | <br/> | ||
| + | -BBa_K875020, Glucosidase (Osaka 2010): | ||
| + | <br/> | ||
| + | |||
Revision as of 21:29, 25 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Data
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Our favourite parts:
-BBa_K875001, T5 cumate operator:
we have dimontrated that it is a very strong and strictly regulated promoter.Thanks to its rapid reactivity it's one of the first candidate for the expression and production of toxin in case of necessity.
-BBa_K875003, CymR: stringenza
we have succesfully expressed the CymR. We have also show his stringency as repressor of the T5 cumate operator that makes the all system a very useful mechanism for an high specificity expression.
-BBa_K875004, LPP-OmpA-scFv:
we have verified the production of this protein at high yield. OmpA is a very powerful method for displaying proteins on the external membrane, in this case the antibody scFv 54.6.
The part we have improved:
-BBa_K875020, Glucosidase (Osaka 2010):
