Team:Bielefeld-Germany/Labjournal/week4

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=Labjournal=
 
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1 Week1]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2 Week2]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3 Week3]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4 Week4]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5 Week5]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6 Week6]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7 Week7]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8 Week8]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9 Week9]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10 Week10]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11 Week11]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12 Week12]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13 Week13]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14 Week14]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15 Week15]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16 Week16]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17 Week17]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18 Week18]
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==Week 4 (05/21 - 05/27/12)==
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===Monday May 21st===
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* '''Team Modeling''': Our aims for modeling:
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<ul style="list-style-type:none">
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/Starting"><strong>Prologue</strong></a></li>
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                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1"><strong>Week 1</strong></a></li>
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                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2"><strong>Week 2</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3"><strong>Week 3</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4"><strong>Week 4</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5"><strong>Week 5</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6"><strong>Week 6</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7"><strong>Week 7</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8"><strong>Week 8</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9"><strong>Week 9</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10"><strong>Week 10</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11"><strong>Week 11</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12"><strong>Week 12</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13"><strong>Week 13</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14"><strong>Week 14</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15"><strong>Week 15</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16"><strong>Week 16</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17"><strong>Week 17</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18"><strong>Week 18</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week19"><strong>Week 19</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week20"><strong>Week 20</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21"><strong>Week 21</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week22"><strong>Week 22</strong></a></li>
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** model the expression of the laccases in the organisms.
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** model the aktivity of our enzyms.
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** model the interesing parts of a clarification plant (the part witch are interesing for our cleaner.
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===Tuesday May 22nd===
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</div>
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* Successful PCRs of laccase genes from ''Thermus thermophilus HB27'' and ''Bacillus halodurans C-125'' with genomic DNA as template. The primers were designed with T7-Promotor-overhanging ends after prefix and HIS-Tag-overhang before suffix.
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==Week 4 (05/21 - 05/27/12)==
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===Wednesday May 23rd===
 
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*''' Team Student Academy:''' Repetition of the transformation of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] with new competent cells.  For setup see [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2#Monday_May_7th here]. Got intense red fluorescing colonies but no green fluorescing colonies. Made a backup of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]. Asking for other plasmids containing GFP at the working groups of our University.
 
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===Thursday May 24th===
 
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*'''Team Student Academy:''' Made a liquid culture of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] at 30°C. There was no fluorescence. Furthermore [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] was transformed. Also got intense red fluorescing colonies.
 
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===Friday May 25th===
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{{Team:Bielefeld/Sponsoren}}
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===Saturday May 26th===
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===Sunday May 27th===
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Latest revision as of 21:12, 25 September 2012


Week 4 (05/21 - 05/27/12)

55px Logo merck.jpg BioCircle.JPG Bielefeld2012 Evonik.jpg Bielefeld2012 Baxter.png Logo knauer.jpg Logo iit.jpg Bielefeld2012 BIEKUBA.jpg Logo biometra.jpg Logo bio-nrw.png Bielefeld2012 Logo ERASynbio.jpg

About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

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