Team:LMU-Munich/Results

From 2012.igem.org

(Difference between revisions)

Revision as of 20:58, 25 September 2012

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

[ more news ]

Main Results banner.jpg

Here you will find the main results of our work.

B. subtilis vectors

Name BioBrick	E. coli res.	B. subt. res.	Insertion locus	Description	Cloning status	Works?
pSB_Bs1C [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823023 (BBa_K823023)]	Amp	Cm	amyE	empty
pSB_Bs4S [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823022 (BBa_K823022)]	Amp	Spec	thrC	empty
pSB_Bs2E [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823027 (BBa_K823027)]	Amp	MLS	lacA	empty	in work	not tested
pSB_Bs1C-lacZ [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823021 (BBa_K823021) ]	Amp	Cm	amyE	lacZ reporter
pSB_Bs3C-luxABCDE [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 (BBa_K823025)]	Amp	Cm	sacA	luxABCDE reporter
pSB_Bs4S-P_Xyl [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823024 (BBa_K823024)]	Amp	Spec	thrC	Xylose-promoter		refuses transformation
pSB_Bs0K-P_spac [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 (BBa_K823026)]	Amp	Kan	replicative	IPTG-promoter		unexpected
Sporovector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 (BBa_K823054)]	Amp	Spec	thrC	to make Sporobeads		not tested

Inverter

Germination Gene Knockouts

The germination rates of B. subtilis germination mutants. All germination rates are relative to wild type 168 (WT168), our positive control. The mutant spo0A::tet is unable to form spores, and should therefore show no germination (our negative control). Mutants used were:
WT168: Wild-type 168
spo0A::tet: A sporulation-deficient strain
B29: cwlD::kan, sleB::mls
B30: gerD::cm, sleB::mls
B32: cwlJ::spec, cwlD::kan
B40: cwlD::kan, sleB::mls, cwlJ::spec
B41: cwlD::kan, sleB::mls, gerD::cm
B42: cwlD::kan, cwlJ::spec, gerD::cm
B43: gerD::cm, sleB::mls, cwlJ::spec
B46: cwlD::kan, cwlJ::spec, gerD::cm, sleB::mls
B47: gerD::cm, sleB::mls, cwlJ::spec, cwlD::kan

For detailed results visit our data page and for all background info our project page.

Project Navigation


Bacillus Intro	Bacillus BioBrickBox	Sporobeads	Germination STOP

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Results"

@@ Line 7: / Line 7: @@
 ===''B. subtilis'' vectors===
 {| class="colored"
-!Name BioBrick
+!Name
+BioBrick
 !''E. coli'' res.
 !''B. subt.'' res.