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Team:Westminster/Notebook
From 2012.igem.org
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<h2>WEEK 1</h2> | <h2>WEEK 1</h2> | ||
<p>The primers for amplification of the ALDH promoter sequences have arrived. These primers have the biobrick overhangs. Primers were diluted and the first PCR reaction to amplify the ALDH promoters was set up and run overnight. Primer stocks were diluted to 100mM and then working concentrations of 10µM were made up. 100µM stocks were produced using the IDT re-suspension calculator. PCR was set up using Pfu polymerase. Template used was whole cells (mammalian). Cells were thawed, spun down and the supernatant removed and then re-suspended in 50uL DMSO. PCR was left to run overnight. Annealing temperature used was 54 degrees, this is slightly higher than the annealing temperature generally used in the lab. </p> | <p>The primers for amplification of the ALDH promoter sequences have arrived. These primers have the biobrick overhangs. Primers were diluted and the first PCR reaction to amplify the ALDH promoters was set up and run overnight. Primer stocks were diluted to 100mM and then working concentrations of 10µM were made up. 100µM stocks were produced using the IDT re-suspension calculator. PCR was set up using Pfu polymerase. Template used was whole cells (mammalian). Cells were thawed, spun down and the supernatant removed and then re-suspended in 50uL DMSO. PCR was left to run overnight. Annealing temperature used was 54 degrees, this is slightly higher than the annealing temperature generally used in the lab. </p> | ||
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<h2>Lorem Ipsum</h2> | <h2>Lorem Ipsum</h2> | ||
Revision as of 20:50, 25 September 2012
WEEK 1
The primers for amplification of the ALDH promoter sequences have arrived. These primers have the biobrick overhangs. Primers were diluted and the first PCR reaction to amplify the ALDH promoters was set up and run overnight. Primer stocks were diluted to 100mM and then working concentrations of 10µM were made up. 100µM stocks were produced using the IDT re-suspension calculator. PCR was set up using Pfu polymerase. Template used was whole cells (mammalian). Cells were thawed, spun down and the supernatant removed and then re-suspended in 50uL DMSO. PCR was left to run overnight. Annealing temperature used was 54 degrees, this is slightly higher than the annealing temperature generally used in the lab.
Lorem Ipsum
Cancer recurrence is one of the fears that almost every patient undergoing chemotherapy develops. Recent findings suggest that only a small fraction of the tumor cells, called Cancer Stem Cells (CSC) are able to drive the growth of the tumor. CSC also show an increased drug resistance, and could remain unaffected after chemotherapy, eventually resulting in the formation of a new tumor.
Lorem Ipsum
Cancer recurrence is one of the fears that almost every patient undergoing chemotherapy develops. Recent findings suggest that only a small fraction of the tumor cells, called Cancer Stem Cells (CSC) are able to drive the growth of the tumor. CSC also show an increased drug resistance, and could remain unaffected after chemotherapy, eventually resulting in the formation of a new tumor.
