Team:Paris-Saclay/Project/Notebook/Week 10

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Revision as of 20:45, 25 September 2012

6th August

Sending of the B1 sample to sequencing with two pairs of primers
- K-274100 Forward and Reverse
- Plasmid pSB1A2 Forward and Reverse

7th August

Liquid culture of B1 in order to prepare a glycerol stock
- Receipt of new primers
- 2 Forward
- 3 Reverse
- Plasmid Reverse

8th August

Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.

PCR program used:

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 {| width="85%"
 |-
-| style="width: 30%;"| Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.
+| style="width: 50%;"| Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.
-| style="width: 70%;"| [[File:Week10-2.jpg|right|330px]]
+| style="width: 35%;"| [[File:Week10-2.jpg|right|330px]]
 |}