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Team:Exeter/lab book/3gip/wk6
From 2012.igem.org
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<p>See Operon Construction: 13th - 17th August 2012</p> | <p>See Operon Construction: 13th - 17th August 2012</p> | ||
| - | <p>https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6</p> | + | <p>https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6</p><br> |
<p><b><u>Wednesday 15th August</u></b></p> | <p><b><u>Wednesday 15th August</u></b></p> | ||
| Line 127: | Line 127: | ||
<p>•<u>Mini-Prepping</u></p> | <p>•<u>Mini-Prepping</u></p> | ||
| - | <p>BBa_J13002 + | + | <p>BBa_J13002 + <i>wbnK</i> BBa_B0014 in pSB1T3</p> |
| - | <p> | + | <p><i>wfcA</i> + BBa_B0014 in pSB1T3</p> |
| - | <p> | + | <p><i>wbnJ</i> + BBa_B0014 in pSB1T3</p> |
| - | <p> | + | <p>BBa_B0034 <i>wbbC</i> + BBa_B0014 in pSB1T3</p> |
<p>Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed. </p> | <p>Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed. </p> | ||
<p>After the neutralisation solution had been added, the eppindorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. </p> | <p>After the neutralisation solution had been added, the eppindorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. </p> | ||
<p>40ul of water was added instead of 40ul in order to make sure the concentration of DNA increased. </p> | <p>40ul of water was added instead of 40ul in order to make sure the concentration of DNA increased. </p> | ||
| - | <p>All were nanodropped and only | + | <p>All were nanodropped and only <i>wfcA</i> + BBa_B0014 and <i>wbnJ</i> + BBa_B0014 gave good concentrations. </p><br> |
<p><b><u>Wednesday 15th August –Friday 17th August</u></b></p> | <p><b><u>Wednesday 15th August –Friday 17th August</u></b></p> | ||
Revision as of 19:54, 25 September 2012
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The 3-Gene Inducible Plasmid: 13th - 17th August 2012 Monday 13th August See Operon Construction: 13th - 17th August 2012 https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6 Tuesday 14th August See Operon Construction: 13th - 17th August 2012 https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6 Wednesday 15th August Morning •Mini-Prepping BBa_J13002 + wbnK BBa_B0014 in pSB1T3 wfcA + BBa_B0014 in pSB1T3 wbnJ + BBa_B0014 in pSB1T3 BBa_B0034 wbbC + BBa_B0014 in pSB1T3 Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed. After the neutralisation solution had been added, the eppindorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. 40ul of water was added instead of 40ul in order to make sure the concentration of DNA increased. All were nanodropped and only wfcA + BBa_B0014 and wbnJ + BBa_B0014 gave good concentrations. Wednesday 15th August –Friday 17th August Preparation of presentation so no lab work. |
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