Team:Hong Kong-CUHK/DOC NBK JUL

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NOTEBOOK - JULY

1. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells.
2.  
3. Plasmids containing NpSRII-NpHtrII-Ectar -backbone gene were sent to sequencing.
4. Make plate with 0.5% soft agar with antibiotics- chloramphenicol.
5. Make plate with antibiotics –Ampiclin.
6. Overlapping PCR of NpSRII-NpHtrII-Ectsr-backbone which the plasmid is responsible in repelling from blue light.
7.  
8. Direct transformation of NpSRII-NpHtrII-Ectsr-backbone gene, follow by spread plate.
9. Miniprep of picked clones from transformed HsSRI-HsHtrI-Ectsr-backbone cells.
10. Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar-backbone gene are incorrect.
11.  
12. Overlapping PCR of NpSRII-NpHtrII-Ectar -backbone which the plasmid is responsible in attracting by blue light.
13. Make LB agar solution
14.  
15. Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate.
16. No clone was being found on the plate containing transformed NpSRII-NpHtrII-Ectar-backbone cells.
17.  
18. Overlapping PCR of NpSRII-NpHtrII-Ectar-backbone which the plasmid is responsible in attracting by blue light.
19. Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate.
20. Pick clones from transformed NpSRII-NpHtrII-Ectar-backbone cells.
21. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells.
22.  
23. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells and was sent for sequencing.
24. Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar -backbone gene is correct.
25. The sequenced cells which contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics.
26.  
27. Repeat in testing cells that contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar     plate for testing using normal light bulb with its corresponding antibiotics.
28. Pick clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells.
29. Plasmids containing HsSRI-HsHtrI-Ectsr -backbone gene were sent to sequencing.
30. Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells.

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