Team:Exeter/lab book/3gip/wk6

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Revision as of 18:11, 25 September 2012

ExiGEM2012 Lab Book 3GP wk6

The 3-Gene Inducible Plasmid: 13th - 17th August 2012

Monday 13th August

See Operon Construction: 13th - 17th August 2012

https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6

Tuesday 14th August

See Operon Construction: 13th - 17th August 2012

https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6

Wednesday 15th August

Morning

•Mini-Prepping

BBa_J13002 + WbnK_BBa_B0014 in pSB1T3

WfcA + BBa_B0014 in pSB1T3

WbnJ + BBa_B0014 in pSB1T3

BBa_B0034_WbbC + BBa_B0014 in pSB1T3

Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed.

After the neutralisation solution had been added, the eppindorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation.

40ul of water was added instead of 40ul in order to make sure the concentration of DNA increased.

All were nanodropped and only WfcA + BBa_B0014 and WbnJ + BBa_B0014 gave good concentrations.

Wednesday 15th August –Friday 17th August

Preparation of presentation so no lab work.

Retrieved from "http://2012.igem.org/Team:Exeter/lab_book/3gip/wk6"