|
|
| (2 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | {{:Team:EPF-Lausanne/Template/Header}} | + | {{:Team:EPF-Lausanne/Template/Header|project}} |
| | | | |
| - | = Illumination =
| |
| - |
| |
| - | In Strickland's paper, it's mentioned that they used 8000 mcd LEDs from [http://www.theledlight.com theledlight.com], with 20º viewing angle and 468 nm at 3.4 V.
| |
| - |
| |
| - | It's also mentioned that a single LED was used per tube, with a cross section of 0.12 cm<sup>2</sup> = 1.2x10<sup>-5</sup> m<sup>2</sup>.
| |
| - |
| |
| - | 20º viewing angle, 0.35 rad, means a solid angle of 2π(1-cos(0.35)) = 0.381 sr.
| |
| - |
| |
| - | This gives a luminous flux of 0.381*8 = 3.05 lm per LED.
| |
| - |
| |
| - | The illuminance they used was then 3.05/1.2x10<sup>-5</sup> = 2.54x10<sup>5</sup> lux (or lm/ m<sup>2</sup>).
| |
| - |
| |
| - | A bioreactor that has some 0.2*π*0.3 = 0.19 m<sup>2</sup>, we would need at least 2.54x10<sup>5</sup> * 0.19 = 48260 lm, or some 16000 LEDs just to make sure the outer surface of the bioreactor has the same light conditions!!
| |
| - |
| |
| - | This doesn't take into account reflections in the bioreactor, so I hope it will lower this number.
| |
| - |
| |
| - | == Illuminance test ==
| |
| - |
| |
| - | We can try first different illuminance values, to get an idea about the range in which the LovTAP-VP16 switch saturates. This could be done by using cylindrical cell culture test plates (there are two sizes: 8.96 sqcm and 3.60 sqcm of circular base).
| |
| - |
| |
| - | If we take the one with 3.60 sqcm (or 2.14 cm in diameter), considering we have 20º LEDs, the distance between the well bottom and the LED has to be of around 6 cm to have the whole well illuminated.
| |
| - |
| |
| - | But, attention! This will be some 20 times less illuminance than in Strickland's experiment!
| |
| - |
| |
| - | If we take the plates with 24 wells, or 1.86 cm2 the well, or 1.54 cm in diameter: the distance from LED to well bottom should be around 4.4 cm. The height of the plates is 22 mm, perfect!
| |
| | | | |
| | == Cell culture absorbance test == | | == Cell culture absorbance test == |
"
