Team:Bonn

From 2012.igem.org

(Difference between revisions)

Revision as of 17:30, 25 September 2012

Home	Team	Project	Activities	Parts Submitted to the Registry	Notebook	Safety	Attributions	Sponsors

This page is currently under construction.

To visit our website at University of Bonn click [http://igem12.uni-bonn.de/en/ HERE].

Fusion protein design has always been time- and design-intensive, to say the least. We are developing and characterizing a fusion construct containing a light sensitive domain, providing easy coupling and light activation of proteins of interest to investigators, thus developing a protein-level light-induced knockout.

Using the LOV (Light, Oxygen, Voltage) domain commonly found in plants, where it enables light-directed growth, we are establishing guidelines for coupling many interesting proteins to the LOV domain, which allows control of protein activity through blue wavelength light.

Designing our reversible knockout at protein level allows quick response times (2.2 microseconds activation time, 85 seconds de- activation time). A device of that kind could be of great importance as a tool for disinfection on a laboratory scale or mutant selection via blue light. Further potential applications of our LOV fusion system might include bioreactor regulation or site-specific drug activation.

iGEM Team Bonn

@@ Line 24: / Line 24: @@
 [[Image:Bonn_lovlogo.png|right|200px]]
-Fusion protein design has always been time- and design-intensive, to say the least. We are developing and characterizing a fusion construct containing a light sensitive domain, providing easy coupling and light activation of proteins of interest for researchers, thus developing a protein-level light-induced knockout.
+Fusion protein design has always been time- and design-intensive, to say the least.
+We are developing and characterizing a fusion construct containing a light sensitive domain,
+providing easy coupling and light activation of proteins of interest to investigators, thus
+developing a protein-level light-induced knockout.
+Using the LOV (Light, Oxygen, Voltage) domain commonly found in plants, where it enables light-directed growth, we are establishing guidelines for coupling many interesting proteins to the LOV domain, which allows control of protein activity through blue wavelength light.
-Using the LOV (Light, Oxygen, Voltage) domain commonly found in some plants, where it enables light-directed growth, we are establishing guidelines for coupling proteins of interest to the LOV domain, which allows control of protein activity through blue wavelength light. Designing our reversible knockout at the protein level allows quick response times (2,2 microseconds activation time, 85 seconds deactivation time).
+Designing our reversible knockout at protein level allows quick response times (2.2 microseconds activation time, 85 seconds de-
+activation time). A device of that kind could be of great importance as a tool for disinfection on a laboratory scale or mutant selection via blue light. Further potential applications of our LOV fusion system might include bioreactor regulation or site-specific drug activation.
-At time our project covers coupling the LOV domain with various proteins which induce cell-death, which allows developing a cell which is killed by light. The experience gained by working on creating the required fusion proteins might make it possible to fuse any other protein with LOV too and make it light-switchable.
 [[Image:Bonn_team.png|right|thumb|iGEM Team Bonn]]