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Team:Cambridge/Parts
From 2012.igem.org
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We contacted the registry about this part and were granted exemption from the pSB1C3 standard for this part. | We contacted the registry about this part and were granted exemption from the pSB1C3 standard for this part. | ||
| - | '''[http://partsregistry.org/wiki/index.php?title=Part: | + | '''[http://partsregistry.org/wiki/index.php?title=Part:BBa_K911001<span style="color:#00000CD"><font size="4">3.Magnesium sensitive riboswitch (Part:BBa_K911001) </font>]''' |
Riboswitch that acts as a regulatory element, truncating transcripts when magnesium is not bound to the RNA. Four Mg2+ binding sites exist, giving this part significantly switch-like behavior. Binding of these sites by Mg2+ results in compaction of the regulatory region of the riboswitch, which in its unbound state acts as an anti-terminator. Loss of this anti-terminator activity results in the activity of a downstream terminator (included in this sequence) which terminates transcription of the gene. May also be affected by other divalent ions, such as manganese. | Riboswitch that acts as a regulatory element, truncating transcripts when magnesium is not bound to the RNA. Four Mg2+ binding sites exist, giving this part significantly switch-like behavior. Binding of these sites by Mg2+ results in compaction of the regulatory region of the riboswitch, which in its unbound state acts as an anti-terminator. Loss of this anti-terminator activity results in the activity of a downstream terminator (included in this sequence) which terminates transcription of the gene. May also be affected by other divalent ions, such as manganese. | ||
| - | '''[http://partsregistry.org/wiki/index.php?title=Part: | + | '''[http://partsregistry.org/wiki/index.php?title=Part:BBa_K911002<span style="color:#00000CD"><font size="4">4.Magnesium sensitive riboswitch (8 codon substitution)(Part:BBa_K911002) </font>]''' |
Riboswitch that acts as a regulatory element, truncating transcripts when magnesium is not bound to the RNA. Four Mg2+ binding sites exist, giving this part significantly switch-like behavior. Binding of these sites by Mg2+ results in compaction of the regulatory region of the riboswitch, which in its unbound state acts as an anti-terminator. Loss of this anti-terminator activity results in the activity of a downstream terminator (included in this sequence) which terminates transcription of the gene. May also be affected by other divalent ions, such as manganese. | Riboswitch that acts as a regulatory element, truncating transcripts when magnesium is not bound to the RNA. Four Mg2+ binding sites exist, giving this part significantly switch-like behavior. Binding of these sites by Mg2+ results in compaction of the regulatory region of the riboswitch, which in its unbound state acts as an anti-terminator. Loss of this anti-terminator activity results in the activity of a downstream terminator (included in this sequence) which terminates transcription of the gene. May also be affected by other divalent ions, such as manganese. | ||
Revision as of 17:08, 25 September 2012
Parts designed and constructed
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K9110031.Fluoride Sensitive Riboswitch (Part:BBa_K911003) ]
Riboswitch that is highly sensitive to the F- ion. We have characterized this part in three different chassis: TOP10 e.coli, Laboratory strain bacillus subtilis and a strain of bacillus subtilis with its normal fluoride riboswitch knocked out (kindly provided by the Breaker lab in Yale). Results of Miller assays for these three chassis are also provided.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K9110042.Synthesised Ratiometric Luciferase construct in non-standard plasmid (Part:BBa_K911004) ]
This part was designed as a ratiometric luciferase reporter. The first promoter, hyperSpank, is LacI - repressed and controls the transcription of a (vibrio harveyi) luxA gene that has been fused at the N-terminus to an mOrange gene via a flexible linker. This was described by Dachuan Ke and Shiao-Chun Tu (2011) as having an additional peak in its emission spectrum at 560 nm, whereas the normal peak is at 490 nm. This is terminated by b0015. Downstream, pVEG controls the translation of the entire normal lux operon, which is again terminated by b0015. The idea is that the normal luciferase output acts as an internal control signal, to which the output of the induced luciferase with the spectral shift can be normalised. We designed this to be compatible with our cheap open-source sensing hardware. We contacted the registry about this part and were granted exemption from the pSB1C3 standard for this part.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K9110013.Magnesium sensitive riboswitch (Part:BBa_K911001) ]
Riboswitch that acts as a regulatory element, truncating transcripts when magnesium is not bound to the RNA. Four Mg2+ binding sites exist, giving this part significantly switch-like behavior. Binding of these sites by Mg2+ results in compaction of the regulatory region of the riboswitch, which in its unbound state acts as an anti-terminator. Loss of this anti-terminator activity results in the activity of a downstream terminator (included in this sequence) which terminates transcription of the gene. May also be affected by other divalent ions, such as manganese.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K9110024.Magnesium sensitive riboswitch (8 codon substitution)(Part:BBa_K911002) ]
Riboswitch that acts as a regulatory element, truncating transcripts when magnesium is not bound to the RNA. Four Mg2+ binding sites exist, giving this part significantly switch-like behavior. Binding of these sites by Mg2+ results in compaction of the regulatory region of the riboswitch, which in its unbound state acts as an anti-terminator. Loss of this anti-terminator activity results in the activity of a downstream terminator (included in this sequence) which terminates transcription of the gene. May also be affected by other divalent ions, such as manganese.
<groupparts>iGEM012 Cambridge</groupparts>
