Team:Exeter/lab book/1gp/wk12

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Revision as of 16:24, 25 September 2012

ExiGEM2012 Lab Book 1GP wk12


Protocols \| Single Gene Plasmids and Enzyme Characterisation \| Showcasing Polysaccharide Production \| The 3-Gene Inducible Plasmid Operon Construction \| Glycobase
9th - 13th July - 16th - 20th July - 23rd - 27th July - 30th July - 3rd August - 6th - 10th August - 13th - 17th August - 20th - 24th August - 27th - 31st August - 3rd - 7th September - 10th - 14th September - 17th - 21st September - 24th - 28th September
	Single Gene Plasmids and Enzyme Characterisation: 24th - 28th September 2012 Monday 24th September (9.00) • Adding cultures with pBAD(weak)+RBS-GFP, pLacI/Ara-1 pLacI/Ara-1+RBS-GFP and pLacI/Ara-1+RBS-WclY-terminator into liquid medium and incubation overnight Uninduced and induced (L-arabinose for pBAD(weak) and IPTG for pLacI/Ara-1) tubes for each of the three constructs were added along with chloramphenicol (for pBAD(weak)+RBS-GFP and pLacI/Ara-1+RBS-GFP) and ampicillin (for pLacI/Ara-1+RBS-WclY-terminator) as the selection antibiotics. pLacI/Ara-1+RBS-WclY-terminator was used to determine basal levels of GFP fluorescence. Tuesday 26th September (9.00) • Mini-Prepping of OmpA • Send off OmpA for sequencing • Transformation of pBAD/AraC promoter weak+RBS-GFP, pBAD/AraC promoter strong+RBS-GFP, pBAD(large)+RBS-GFP, constitutive promoter+RBS-GFP, pLacI/Ara-1+RBS-GFP, WclY-pSB1C3, WbnJ-pSB1C3, WbbC-pSB1C3, WfcA-terminator and RBS-WbiP Wednesday 27th September (9.00) • Mini-Prepping of OmpA • Send off OmpA for sequencing • Transformation of pBAD/AraC promoter weak+RBS-GFP, pBAD/AraC promoter strong+RBS-GFP, pBAD(large)+RBS-GFP, constitutive promoter+RBS-GFP, pLacI/Ara-1+RBS-GFP, WclY-pSB1C3, WbnJ-pSB1C3, WbbC-pSB1C3, WfcA-terminator and RBS-WbiP

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      <p><u><b>Monday 24th September (9.00)</u></b></p>
-<p>• <a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#1d1d1b"><u>Mini-Prepping</u></a> of OmpA</p>
+<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#1d1d1b"><u>Adding cultures</u></a> with pBAD(weak)+RBS-GFP, pLacI/Ara-1 pLacI/Ara-1+RBS-GFP and pLacI/Ara-1+RBS-WclY-terminator into liquid medium and incubation overnight
-<p>• Send off OmpA for sequencing</p>
+<p> Uninduced and induced (L-arabinose for pBAD(weak) and IPTG for pLacI/Ara-1) tubes for each of the three constructs were added along with chloramphenicol (for pBAD(weak)+RBS-GFP and pLacI/Ara-1+RBS-GFP) and ampicillin (for pLacI/Ara-1+RBS-WclY-terminator) as the selection antibiotics. pLacI/Ara-1+RBS-WclY-terminator was used to determine basal levels of GFP fluorescence.</p>
-<p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#1d1d1b"><u>Transformation</u></a> of pBAD/AraC promoter weak+RBS-GFP, pBAD/AraC promoter strong+RBS-GFP, pBAD(large)+RBS-GFP, constitutive promoter+RBS-GFP, pLacI/Ara-1+RBS-GFP, WclY-pSB1C3, WbnJ-pSB1C3, WbbC-pSB1C3, WfcA-terminator and RBS-WbiP </p>
 <p><u><b>Tuesday 26th September (9.00)</u></b></p>