Team:Groningen/Notebook/Wetwork 4August2012
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Transformation of E.coli DH5a with BBa_R0011 succeeded! They were grown in liquid LB culture today. | Transformation of E.coli DH5a with BBa_R0011 succeeded! They were grown in liquid LB culture today. | ||
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+ | Emeraldo | ||
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+ | PCR reaction with Phusion polymerase: | ||
+ | 1.) Violacein PCR with BBa_K274002 as template --> 7,3kb band detected (OK!) | ||
+ | 2.) Lycopene PCR with BBa_K274100 as template --> 3,4kb band detected (OK!) | ||
Revision as of 13:22, 25 September 2012
Transformation of E.coli DH5a with BBa_R0011 succeeded! They were grown in liquid LB culture today.
Emeraldo
PCR reaction with Phusion polymerase: 1.) Violacein PCR with BBa_K274002 as template --> 7,3kb band detected (OK!) 2.) Lycopene PCR with BBa_K274100 as template --> 3,4kb band detected (OK!)