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Team:Exeter/lab book/1gp/wk12
From 2012.igem.org
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| - | + | <p><u><b>Monday 24th September (9.00)</u></b></p> | |
| + | <p>• <a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#1d1d1b"><u>Mini-Prepping</u></a> of OmpA</p> | ||
| + | <p>• Send off OmpA for sequencing</p> | ||
| + | <p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#1d1d1b"><u>Transformation</u></a> of pBAD/AraC promoter weak+RBS-GFP, pBAD/AraC promoter strong+RBS-GFP, pBAD(large)+RBS-GFP, constitutive promoter+RBS-GFP, pLacI/Ara-1+RBS-GFP, WclY-pSB1C3, WbnJ-pSB1C3, WbbC-pSB1C3, WfcA-terminator and RBS-WbiP </p> | ||
| + | |||
| + | <p><u><b>Tuesday 26th September (9.00)</u></b></p> | ||
| + | <p>• <a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#1d1d1b"><u>Mini-Prepping</u></a> of OmpA</p> | ||
| + | <p>• Send off OmpA for sequencing</p> | ||
| + | <p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#1d1d1b"><u>Transformation</u></a> of pBAD/AraC promoter weak+RBS-GFP, pBAD/AraC promoter strong+RBS-GFP, pBAD(large)+RBS-GFP, constitutive promoter+RBS-GFP, pLacI/Ara-1+RBS-GFP, WclY-pSB1C3, WbnJ-pSB1C3, WbbC-pSB1C3, WfcA-terminator and RBS-WbiP </p> | ||
| + | |||
| + | <p><u><b>Wednesday 27th September (9.00)</u></b></p> | ||
| + | <p>• <a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#1d1d1b"><u>Mini-Prepping</u></a> of OmpA</p> | ||
| + | <p>• Send off OmpA for sequencing</p> | ||
| + | <p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#1d1d1b"><u>Transformation</u></a> of pBAD/AraC promoter weak+RBS-GFP, pBAD/AraC promoter strong+RBS-GFP, pBAD(large)+RBS-GFP, constitutive promoter+RBS-GFP, pLacI/Ara-1+RBS-GFP, WclY-pSB1C3, WbnJ-pSB1C3, WbbC-pSB1C3, WfcA-terminator and RBS-WbiP </p> | ||
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Revision as of 12:40, 25 September 2012
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Single Gene Plasmids and Enzyme Characterisation: 24th - 28th September 2012 Monday 24th September (9.00) • Mini-Prepping of OmpA • Send off OmpA for sequencing • Transformation of pBAD/AraC promoter weak+RBS-GFP, pBAD/AraC promoter strong+RBS-GFP, pBAD(large)+RBS-GFP, constitutive promoter+RBS-GFP, pLacI/Ara-1+RBS-GFP, WclY-pSB1C3, WbnJ-pSB1C3, WbbC-pSB1C3, WfcA-terminator and RBS-WbiP Tuesday 26th September (9.00) • Mini-Prepping of OmpA • Send off OmpA for sequencing • Transformation of pBAD/AraC promoter weak+RBS-GFP, pBAD/AraC promoter strong+RBS-GFP, pBAD(large)+RBS-GFP, constitutive promoter+RBS-GFP, pLacI/Ara-1+RBS-GFP, WclY-pSB1C3, WbnJ-pSB1C3, WbbC-pSB1C3, WfcA-terminator and RBS-WbiP Wednesday 27th September (9.00) • Mini-Prepping of OmpA • Send off OmpA for sequencing • Transformation of pBAD/AraC promoter weak+RBS-GFP, pBAD/AraC promoter strong+RBS-GFP, pBAD(large)+RBS-GFP, constitutive promoter+RBS-GFP, pLacI/Ara-1+RBS-GFP, WclY-pSB1C3, WbnJ-pSB1C3, WbbC-pSB1C3, WfcA-terminator and RBS-WbiP |
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