Team:Tsinghua/Parts

From 2012.igem.org

(Difference between revisions)
(Prototype team page)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 +
<title>无标题文档</title>
 +
<script src="http://ajax.googleapis.com/ajax/libs/jquery/1.8.0/jquery.min.js">
 +
</script>
 +
<style type="text/css">
 +
/*hack*/
-
<html>
+
body{
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
background-color:#FFF;
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
}
-
This is a template page. READ THESE INSTRUCTIONS.
+
#content{
-
</div>
+
border:none;
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
}
-
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
#catlinks{
-
</div>
+
visibility:hidden;
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
}
-
You <strong>MUST</strong>  have all of the pages listed in the menu below with the names specified.  PLEASE keep all of your pages within your teams namespace. 
+
.right-menu li a {
-
</div>
+
background-color:transparent;
-
</div>
+
-
</html>
+
-
<!-- *** End of the alert box *** -->
 
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
}
-
!align="center"|[[Team:Tsinghua|Home]]
+
-
!align="center"|[[Team:Tsinghua/Team|Team]]
+
.firstHeading{visibility:hidden}
-
!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Tsinghua Official Team Profile]
+
-
!align="center"|[[Team:Tsinghua/Project|Project]]
+
-
!align="center"|[[Team:Tsinghua/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:Tsinghua/Modeling|Modeling]]
+
-
!align="center"|[[Team:Tsinghua/Notebook|Notebook]]
+
-
!align="center"|[[Team:Tsinghua/Safety|Safety]]
+
-
!align="center"|[[Team:Tsinghua/Attributions|Attributions]]
+
-
|}
+
 +
/*hack*/
 +
a{
 +
text-decoration:none;
 +
color:#000000;
 +
}
 +
a:hover{
 +
text-decoration:none;
 +
color:#000000;
 +
}
 +
a:link{
 +
}
 +
a:active{
 +
}
 +
a:visited{
 +
}
 +
div#BannerCleaner{
 +
position:absolute;
 +
top:-99px;
 +
left:0px;
 +
z-index:1;
 +
width:1000px;
 +
height:2000px;
 +
border:none;
 +
background:#FFFFFF;
 +
display:block;
 +
}
 +
div#Header{
 +
position:absolute;
 +
z-index:2;
 +
top:-99px;
 +
left:0px;
 +
width:977px;
 +
height:306px;
 +
background:#32B4EB;
 +
background-image:url("https://static.igem.org/mediawiki/2012/0/03/Part_banner.gif");
 +
background-repeat:no-repeat;
 +
}
 +
div.buttom{
 +
position:absolute;
 +
width:196px;
 +
height:73px;
 +
display:block;
 +
background:#C3C;
 +
cursor:pointer;
 +
overflow:hidden;
 +
z-index:2;
 +
}
 +
div.buttomHome{
 +
position:absolute;
 +
left: 433px;
 +
top: 217px;
 +
width: 110px;
 +
height: 157px;
 +
display:block;
 +
background:#C3C;
 +
cursor:pointer;
 +
overflow:hidden;
 +
z-index:2;
 +
 +
}
 +
div.buttom:hover{
 +
position:absolute;
 +
width:196px;
 +
height:73px;
 +
display:block;
 +
background:#C3C;
 +
opacity:0.7;
 +
filter:alpha(opacity = 70);
 +
overflow:hidden;
 +
cursor:pointer;
 +
}
 +
div.buttomHome:hover{
 +
position:absolute;
 +
left: 433px;
 +
top: 217px;
 +
width: 110px;
 +
height: 157px;
 +
display:block;
 +
background:#C3C;
 +
opacity:0.7;
 +
filter:alpha(opacity = 70);
 +
overflow:hidden;
 +
cursor:pointer;
 +
}
 +
div.buttom img{
 +
position:relative;
 +
width:100%;
 +
height:100%;
 +
}
 +
div.buttomHome img{
 +
position:relative;
 +
width:100%;
 +
height:100%;
 +
}
 +
.content{
 +
position:absolute;
 +
top:458px;
 +
left:0px;
 +
width:100%;
 +
height:600px;
 +
display:block;
 +
z-index:2;
 +
}
 +
#ground{
 +
width:100%;
 +
height:800px;
 +
background:#FFFFFF;
 +
}
 +
.contentPanel{
 +
position:absolute;
 +
padding-left:12px;
 +
padding-right:12px;
 +
padding-top:20px;
 +
padding-bottom:20px;
 +
left: 180px;
 +
top: 4px;
 +
width: 831px;
 +
height: auto;
 +
display:none;
 +
font-family:Tahoma, Geneva, sans-serif;
 +
font-size:11pt;
 +
color:#666;
 +
}
 +
.projectList{
 +
padding-top: 20px;
 +
position: fixed;
 +
top: 379px;
 +
height: 173px;
 +
width: 206px;
 +
left: 8px;
 +
border:1px solid;
 +
background-color:#FF9;
 +
border-color:#F93;
 +
border-radius:10px;
 +
-moz-border-radius:10px; /* Firefox 3.6 and earlier */
 +
z-index:99;
 +
}
 +
#navi{
-
An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
+
list-style:none;
 +
padding:0px;
 +
margin:0px;
 +
}
 +
#navi li{
 +
margin-left:2px;
 +
font-family:Tahoma, Geneva, sans-serif}
 +
#navi li ul{
 +
list-style:none;
 +
padding:0px;
 +
margin:0px;
 +
}
 +
#navi li ul li{
 +
list-style:circle;
 +
}
 +
#navi ul ul{
 +
list-style:disc;
 +
}
 +
li:hover{
 +
background:#66CCFF;
 +
cursor:pointer;
 +
}
 +
.parag{
 +
position: absolute;
 +
left: 243px;
 +
top: 379px;
 +
width: 739px;
 +
height: 600px;
 +
z-index:3;
 +
}
 +
p{font-family:Arial, Helvetica, sans-serif}
-
Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
 
 +
</style>
 +
<script type="text/javascript">
 +
$("document").ready(function(){
 +
$("div[pID=1]").click(function(){
 +
 +
$(this).animate({left:'-=2', top:'-=2',width:'+=4',height:'+=4'}, 'fast');
 +
$(this).animate({left:'+=2', top:'+=2',width:'-=4',height:'-=4'}, 'fast');
-
<groupparts>iGEM012 Tsinghua</groupparts>
+
});
 +
});
 +
 
 +
 
 +
var currentContent = 1
 +
$("document").ready(function(){
 +
$("#contentPanel" + 1).fadeIn('slow');
 +
});
 +
 
 +
function showContent(number){
 +
if (number != currentContent){
 +
$("div[id*='contentPanel']").fadeOut('slow');
 +
}
 +
$("#contentPanel" + number).fadeIn('slow');
 +
currentContent = number;
 +
 +
}
 +
</script>
 +
</head>
 +
 
 +
<body>
 +
<div id = "ground"></div>
 +
<div id = "BannerCleaner"></div>
 +
<div class="Header" id="Header"></div>
 +
<div id = "Home" class = "buttomHome" pID = 1 flag1 = 0><a href="https://2012.igem.org/Team:Tsinghua"><img src="https://static.igem.org/mediawiki/2012/4/43/Home_tsinghua.gif"/></a></div>
 +
<div id = "Project" class = "buttom" style = "top:217px; left:1px;" pID = 1 flag1 = 0><a href="https://2012.igem.org/Team:Project"><img src = "https://static.igem.org/mediawiki/2012/3/37/Project_tsinghua.gif"></a></div>
 +
<div id = "Dataresult" class = "buttom" style = "top:217px; left:215px;" pID = 1 flag1 = 0><a href="https://2012.igem.org/Team:Tsinghua/Dataresult"><img src = "https://static.igem.org/mediawiki/2012/8/8a/Data.gif"></a></div>
 +
<div id = "Team" class = "buttom" style = "top:217px; left:568px;" pID = 1 flag1 = 0><a href="https://2012.igem.org/Team:Tsinghua/Team"><img src = "https://static.igem.org/mediawiki/2012/a/a7/Team_tsinghua.gif"></a></div>
 +
<div id = "Safety" class = "buttom" style = "top:217px; left:782px;" pID = 1 flag1 = 0><a href="https://2012.igem.org/Team:Tsinghua/Safety"><img src = "https://static.igem.org/mediawiki/2012/6/66/Safety_tsinghua.gif"></a></div>
 +
 
 +
<div id = "Notebook" class = "buttom" style = "top:302px; left:1px;" pID = 1 flag1 = 0><a href="https://2012.igem.org/Team:Tsinghua/Notebook"><img src = "https://static.igem.org/mediawiki/2012/4/49/Notebook_tsinghua.gif"/></a></div>
 +
<div id = "Model" class = "buttom" style = "top:302px; left:215px;" pID = 1 flag1 = 0><a href="https://2012.igem.org/Team:Tsinghua/Model"><img src = "https://static.igem.org/mediawiki/2012/8/8e/Model_tsinghua.gif"></a></div>
 +
<div id = "Humanpractice" class = "buttom" style = "top:302px; left:569px;" pID = 1 flag1 = 0><a href="https://2012.igem.org/Team:Tsinghua/Humanpractice"><img src = "https://static.igem.org/mediawiki/2012/c/ca/HumanPractice.gif"/></a></div>
 +
<div id = "Parts" class = "buttom" style = "top:302px; left:782px;" pID = 1 flag1 = 0><a href="https://2012.igem.org/Team:Tsinghua/Parts"><img src = "https://static.igem.org/mediawiki/2012/b/b5/Parts_tsinghua.gif"></a></div>
 +
<div id = "contentPanel1" class = "contentPanel">
 +
</div>
 +
 
 +
<div class="projectList" id="projectList" style="padding-left:20px">
 +
<ul id = "navi">
 +
<li><a href="#PlacLP">BBa_K766001</a></li>
 +
<li><a href="#RFPrt">BBa_K766002</a></li>
 +
<li><a href="#3">BBa_K766003</a></li>
 +
<li><a href="#6000">BBa_K766000</a></li>
 +
<li><a href="#5">BBa_K766005</a></li>
 +
<li><a href="#6">BBa_K766006</a></li>
 +
<li><a href="#7">BBa_K766007</a></li>
 +
<li><a href="#4">BBa_K766004</a></li>
 +
 +
</ul>
 +
</ul>
 +
</ul></div>
 +
 
 +
<div class = "parag">
 +
  <div class = "figure" style="padding-left:20px;">
 +
    <p align="center" style="font-size:18px"><strong>2012 iGEM Parts by Tsinghua</strong></p>
 +
    <p align="center">&nbsp;</p>
 +
    <p><a name="PlacLP"><strong>1.Plac-LuxR-Plux</strong></a><br />
 +
      <a href="http://partsregistry.org/Part:BBa_K766001" style="color:#F00">BBa_K766001</a><br />
 +
      There is something wrong with the  previous part BBa_J09855, which doesn't work well. After we sequenced the part,  we find that the sequence of Plac promoter and Plux promoter is incorrect. So  that we rebuild this part using overlap PCR and DNA systhesis. Finally, our  part can work normally.<br />
 +
  <a href="http://partsregistry.org/Part:BBa_K766001"></a><br />
 +
  <img src="https://static.igem.org/mediawiki/igem.org/f/f1/Part_1.jpg" alt="" width="453" height="265" border="0" /></p>
 +
    <p><a name="RFPrt"><strong>2.RFP with RBS and T1 terminator</strong></a><br />
 +
      <a href="http://partsregistry.org/Part:BBa_K766002" style="color:#F00">BBa_K766002</a><br />
 +
      We build this part as a reporter to  detect the activation of upstream promoter. This fluorescence protein is very  easily detectable even by eyes. Besides, we have added a strong RBS and T1  terminator to the end. You can use this part to construct a more complicated  part further more.<br />
 +
      <br />
 +
  <img src="https://static.igem.org/mediawiki/igem.org/f/fe/Part2.jpg" alt="" width="512" height="272" border="0" /></p>
 +
    <p><a name="3"><strong>3. RFP with RBS and T1 terminator</strong></a><br />
 +
      <a href="http://partsregistry.org/Part:BBa_K766003" style="color:#F00">BBa_K766003</a><br />
 +
    We  build this part to work as a receiver. C6HSL from the neighbor cell can diffuse  across the membrane and activate the transcription from Plux promoter. There is  something wrong with original Part:BBa_J09855. So we rebuild the reporter part  which can be activated by AHL and add the reporter part RFP which is easily  detectable. The expression of it is very clear.</p>
 +
    <p> <img src="https://static.igem.org/mediawiki/igem.org/8/80/Part3.jpg" alt="" width="554" height="222" border="0" /><br />
 +
      <a href="http://partsregistry.org/Part:BBa_K766003"></a></p>
 +
    <p>&nbsp;</p>
 +
    <p><a name="6000"><strong>4. His-Tag-LuxI without LVA-tag</strong></a><br />
 +
      <a href="http://partsregistry.org/Part:BBa_K766000" style="color:#F00">BBa_K766000</a><br />
 +
      This  part is a protein coding sequence which encodes a LuxI protein without LVA-tag  and added a His-tag at the amino terminus. As a result, not only can this LuxI  be degraded at a shorter time but it can be purified by affinity  chromatography.<br />
 +
  <a href="http://partsregistry.org/Part:BBa_K766000"></a></p>
 +
    <p>&nbsp;</p>
 +
    <p align="center"><img src="https://static.igem.org/mediawiki/igem.org/7/74/Part4.jpg" alt="" width="344" height="206" border="0" /></p>
 +
    <p align="center">&nbsp;</p>
 +
    <p><a name="5"><strong>5. T7 promoter-His tag-LuxI</strong></a><br />
 +
      <a href="http://partsregistry.org/Part:BBa_K766005" style="color:#F00">BBa_K766005</a><br />
 +
      We build this part to work as the  signal sender in our project. T7 promoter is a high-efficient promoter which  can bind with T7 polymerase and start the transcription. Besides, we add the  lac operator between T7 promoter and CDS. If you use this part in a luxI-coding  plasmid like pet15b, the expression of gene will be repressed. However, IPTG  can be used to activate the expression. His tag is on the N-terminal, which  consists of six histidine amino acid. We can use Ni-column to purify the  protein. Besides, you can use Hig-tag antibody to detect the protein in western  blot. This design makes the detection of luxI gene become very easy.<br />
 +
  <a href="http://partsregistry.org/Part:BBa_K766005"></a></p>
 +
    <p><img src="https://static.igem.org/mediawiki/igem.org/2/24/Part5.jpg" alt="" width="529" height="208" border="0" /><br />
 +
      <img src="https://static.igem.org/mediawiki/igem.org/2/2b/Part_clip_image012.jpg" alt="" width="554" height="287" border="0" /></p>
 +
    <p align="center">&nbsp;</p>
 +
    <p><a name="6"><strong>6. Plux-LacI without LVA-tag</strong></a><br />
 +
    <a href="http://partsregistry.org/Part:BBa_K766006" style="color:#F00">BBa_K766006</a></p>
 +
    <p>         This  device is a protein-expression unit which contains a Plux promoter and a  transcriptional unit of LacI without LVA-tag. Plux can be activated by luxR-AHL  complex and the promoter can activate the expression of downstream genes. We  add LacI in this part. As we all know, LacI is a repressor which can bind with  Lac operator. With the help of this feature, many logic gates or gene  expression modulation can be achieved.</p>
 +
    <p>There is not LVA-tag in this part so that  the repressor can keep working for a relatively long time.</p>
 +
    <p><a href="http://partsregistry.org/Part:BBa_K766006"></a></p>
 +
    <p align="center"><img src="https://static.igem.org/mediawiki/igem.org/9/92/Part6.jpg" alt="" width="318" height="212" border="0" /></p>
 +
    <p align="center">&nbsp;</p>
 +
    <p align="center">&nbsp;</p>
 +
    <p><a name="7"><strong>7. Plas/cI hybrid Promoter-RFP</strong></a><br />
 +
      <a href="http://partsregistry.org/Part:BBa_K766007" style="color:#F00">BBa_K766007</a><br />
 +
      This  device is composed of a Plac/cI hybrid promoter and a translational unit of  mRFP. The Plas/cI hybrid promoter is made up of Plas promoter and cI operator. The  promoter can be activated by the LasR-C12HSL complex and repressed by cI  repressor. This part can be used in building some logic gates. The RFP segment  is used as a signal to show the result of activation or repression. This kind  fluorescent protein is very easy to be detected even by eyes.<br />
 +
  <a href="http://partsregistry.org/Part:BBa_K766007"></a></p>
 +
    <p align="center"><img src="https://static.igem.org/mediawiki/igem.org/f/fe/Part7.jpg" alt="" width="453" height="227" border="0" /></p>
 +
    <p>&nbsp;</p>
 +
    <p><a name="4"><strong>8. AndNot gate based on AHL</strong></a><br />
 +
      <a href="http://partsregistry.org/Part:BBa_K766004" style="color:#F00">BBa_K766004</a><br />
 +
 +
    <p>We design this A and not B gate. This  part is based on the two kinds of AHL：C6HSL (signal A) and C12HSL(signal B). C6HSL can activate the  promoter Plux and C12HSL can activate the promoter Plas. In this part, we add  cI operator downstream of Plas. As a result, the promoter Plas can be activated  by signal B but repressed by cI protein. The expression of cI protein is under  the control of Plux, which can be activated by signal A. RFP is controlled by  Hybrid promoter (Plas and cI operator), which will work as a reporter. <br />
 +
      In a word, the expression of RFP is  only on the condition that A is absent and B is present. <br />
 +
  <img src="https://static.igem.org/mediawiki/igem.org/b/bf/Part8.jpg" alt="" width="553" height="184" border="0" /></p>
 +
  </div>
 +
<div class = "figure">
 +
    <p>&nbsp;</p>
 +
  </div>
 +
  <div class="figure"></div>
 +
    <div class="figure"></div>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <div class= "figure"></div>
 +
  <p> </p>
 +
<p>&nbsp;</p>
 +
</div>
 +
</div>
 +
 
 +
 
 +
</body>
 +
</html>

Revision as of 12:25, 25 September 2012

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> 无标题文档

2012 iGEM Parts by Tsinghua

 

1.Plac-LuxR-Plux
BBa_K766001
There is something wrong with the previous part BBa_J09855, which doesn't work well. After we sequenced the part, we find that the sequence of Plac promoter and Plux promoter is incorrect. So that we rebuild this part using overlap PCR and DNA systhesis. Finally, our part can work normally.

2.RFP with RBS and T1 terminator
BBa_K766002
We build this part as a reporter to detect the activation of upstream promoter. This fluorescence protein is very easily detectable even by eyes. Besides, we have added a strong RBS and T1 terminator to the end. You can use this part to construct a more complicated part further more.

3. RFP with RBS and T1 terminator
BBa_K766003
We build this part to work as a receiver. C6HSL from the neighbor cell can diffuse across the membrane and activate the transcription from Plux promoter. There is something wrong with original Part:BBa_J09855. So we rebuild the reporter part which can be activated by AHL and add the reporter part RFP which is easily detectable. The expression of it is very clear.


 

4. His-Tag-LuxI without LVA-tag
BBa_K766000
This part is a protein coding sequence which encodes a LuxI protein without LVA-tag and added a His-tag at the amino terminus. As a result, not only can this LuxI be degraded at a shorter time but it can be purified by affinity chromatography.

 

 

5. T7 promoter-His tag-LuxI
BBa_K766005
We build this part to work as the signal sender in our project. T7 promoter is a high-efficient promoter which can bind with T7 polymerase and start the transcription. Besides, we add the lac operator between T7 promoter and CDS. If you use this part in a luxI-coding plasmid like pet15b, the expression of gene will be repressed. However, IPTG can be used to activate the expression. His tag is on the N-terminal, which consists of six histidine amino acid. We can use Ni-column to purify the protein. Besides, you can use Hig-tag antibody to detect the protein in western blot. This design makes the detection of luxI gene become very easy.


 

6. Plux-LacI without LVA-tag
BBa_K766006

         This device is a protein-expression unit which contains a Plux promoter and a transcriptional unit of LacI without LVA-tag. Plux can be activated by luxR-AHL complex and the promoter can activate the expression of downstream genes. We add LacI in this part. As we all know, LacI is a repressor which can bind with Lac operator. With the help of this feature, many logic gates or gene expression modulation can be achieved.

There is not LVA-tag in this part so that the repressor can keep working for a relatively long time.

 

 

7. Plas/cI hybrid Promoter-RFP
BBa_K766007
This device is composed of a Plac/cI hybrid promoter and a translational unit of mRFP. The Plas/cI hybrid promoter is made up of Plas promoter and cI operator. The promoter can be activated by the LasR-C12HSL complex and repressed by cI repressor. This part can be used in building some logic gates. The RFP segment is used as a signal to show the result of activation or repression. This kind fluorescent protein is very easy to be detected even by eyes.

 

8. AndNot gate based on AHL
BBa_K766004

We design this A and not B gate. This part is based on the two kinds of AHL：C6HSL (signal A) and C12HSL(signal B). C6HSL can activate the promoter Plux and C12HSL can activate the promoter Plas. In this part, we add cI operator downstream of Plas. As a result, the promoter Plas can be activated by signal B but repressed by cI protein. The expression of cI protein is under the control of Plux, which can be activated by signal A. RFP is controlled by Hybrid promoter (Plas and cI operator), which will work as a reporter.
In a word, the expression of RFP is only on the condition that A is absent and B is present.

 

 

 

 

 

Retrieved from "http://2012.igem.org/Team:Tsinghua/Parts"