"
Team:TU Munich/Notebook/Labjournal
From 2012.igem.org
(Difference between revisions)
VolkerMorath (Talk | contribs) (→PCR reaction batch) |
VolkerMorath (Talk | contribs) (→Quick Change mutagenis to remove NgoMIV from pYES2) |
||
| Line 2: | Line 2: | ||
===Quick Change mutagenis to remove NgoMIV from pYES2=== | ===Quick Change mutagenis to remove NgoMIV from pYES2=== | ||
'''Investigator: Ingmar, Volker''' | '''Investigator: Ingmar, Volker''' | ||
| + | Aim of the experiment: Generation of an RFC 25 compatible version of the pYes2 Vector. | ||
| + | == PCR reaction batch == | ||
{|cellspacing="0" border="1" | {|cellspacing="0" border="1" | ||
| - | |||
| - | |||
| - | |||
|'''volume''' | |'''volume''' | ||
|'''reagent''' | |'''reagent''' | ||
Revision as of 15:43, 23 June 2012
Saturday, 23th June
Quick Change mutagenis to remove NgoMIV from pYES2
Investigator: Ingmar, Volker Aim of the experiment: Generation of an RFC 25 compatible version of the pYes2 Vector.
PCR reaction batch
| volume | reagent |
| 2.5 µl | 10x Pfu Ultra II buffer |
| 4 µl | Plasmid P7 pYes2_RFC25 MCS template |
| 0.5 µl | 1:10 dilution of O38 (10 pmol/µL) |
| 0.5 µl | 1:10 dilution of O39 ((10 pmol/µL) |
| 17 µl | ddH2O |
| 0.5 µl | dNTP mix |
| 0.5 µl | Pfu Turbo DNA polymerase (2.5 U / µl) |
