Team:MIT/Results

From 2012.igem.org

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<b> Strategy 1: Take DNA sequences that worked in In Vitro Strand Displacement, convert to RNA, transfect inside HEK293 cells </b>
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<b> Strategy 1: Lipofectamine 2000 Transfection of RNA version of Reporter from Winfree/QIan 2011 Paper</b>
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<b> Strategy 2: Tag RNA strand with an Alexa Fluorophore to act as a transfection marker </b>
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<br> Strategy 2: Switch Transfection reagent to RNAiMAX </b>
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<b> Strategy 3: Tag RNA strand with an Alexa Fluorophore to act as a transfection marker </b>
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<b> Strategy 3: Create DNA plasmids driving transcription of RNA inputs, while transfecting RNA Reporter </b>
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<b> Strategy 4: Create DNA plasmids driving transcription of RNA inputs, while transfecting RNA Reporter </b>
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<b> [[Strategy 5]]: Redesign RNA Reporter </b>
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<b> [Strategy 5]: Redesign RNA Reporter </b>
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Revision as of 04:50, 25 September 2012

iGEM 2012

Overview

  • Placeholder

Biobricks

  • Placeholder

Foundational

  • In Vitro Strand Displacement
  • Nucleic Acid Delivery
  • In Vivo Strand Displacement

Sensing

  • Modeling
  • In Vitro Sensing

Processing

  • Modeling
  • In Vitro Not Gate
  • Hammerhead Ribozymes

Actuation

  • Modeling
  • In Vitro
  • In Vivo
RNA Strand Displacement In Vitro

Nucleic Acid Delivery

In Vivo RNA Strand Displacement

Strategy 1: Lipofectamine 2000 Transfection of RNA version of Reporter from Winfree/QIan 2011 Paper


Strategy 2: Switch Transfection reagent to RNAiMAX
Strategy 3: Tag RNA strand with an Alexa Fluorophore to act as a transfection marker

Strategy 4: Create DNA plasmids driving transcription of RNA inputs, while transfecting RNA Reporter

Strategy 4: Nucleofect RNA reporter, RNA inputs

[Strategy 5]: Redesign RNA Reporter

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