Team:XMU-China/protocolsindex

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Revision as of 22:51, 24 September 2012

protocolsindex

Contents[hide] [show]
1 General protocols
1.1 Stock solution
1.2 Preparation of Competent BL21
1.3 Transformation
1.4 Plasmid Purification
1.5 Reaction system of restriction endonuclease
1.6 Standard BioBrick Assembly
1.7 Suffix Insertion
1.8 Ligation
1.9 Restriction analysis
1.10 Gel Extraction
2 Characterization
2.1 Fluorescence Measurements
2.2 Protein electrophoresis
2.2.1 Preparation of Samples
2.2.2 Manufacture Albumen Gel
2.2.3 Electrophoresis
2.2.4 Dyeing (Colloidal Coomassie Brilliant Blue)
2.2.5 Scanning
3 Immobilization
3.1 Prepare Sodiumcellu-losesulfate (NaCS)
3.2 Prepare microcapsules
3.3 Immobilize cells into calcium alginate beads
3.4 Immobilize cells into intra-hollow Ca-alginate capsules
3.5 Immobilize cells into NaCS-PDMDAAC microcapsules

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