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| - | + | After doing a PCR, the resulting DNA should be cleaned up to get | |
| + | rid of the primers, the enzymes and the various other reagents | ||
| + | used in the PCR. | ||
| + | |||
| + | We used Macherey-Nagel's "Nucleospin®istered; Gel and PCR clean-up" kit. | ||
| + | Its manual can be found here: [[http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/DNA%20clean-up/UM_PCRcleanup_Gelex_NSGelPCR.pdf]] | ||
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After doing a PCR, the resulting DNA should be cleaned up to get
rid of the primers, the enzymes and the various other reagents
used in the PCR.
We used Macherey-Nagel's "Nucleospin®istered; Gel and PCR clean-up" kit. Its manual can be found here: http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/DNA clean-up/UM_PCRcleanup_Gelex_NSGelPCR.pdf
"
