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Team:Trieste/notebook7
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| - | + | To get a proof of pelB-scFv and LPP-OmpA-scFv expression, we used standard techniques as SDS-PAGE and Western blotting. The results showed that in both cases scFv is expressed. LPP-OmpA is expressed in great quantities. PelB-scFv is present only in traces. We have suspects that pelB-Ab is toxic for this E. coli strain. | |
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Revision as of 17:45, 24 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Week 7
More
Suicide System
We received the sequencing results of RBS_B0031-Tse2-TT_B0015 construct and double checked before the BB submission. At this point we were able to clone it downstream the T5LacOperator Promoter (BBa_K875002) to test its functionality. We screened the colonies and two of them resulted positive (T5LacOp-B0031-Tse2-B0015), so we plated them with and without IPTG. One of the two colonies doesn't grow on IPTG and grew without it! We also did the same test in LB culture and the same colony died with IPTG and grew without it. We also recorded the OD of the LB culture IPTG induced and not induced at fixed time intervals for several hours during the day. We designed the growth curve.Antibody
To get a proof of pelB-scFv and LPP-OmpA-scFv expression, we used standard techniques as SDS-PAGE and Western blotting. The results showed that in both cases scFv is expressed. LPP-OmpA is expressed in great quantities. PelB-scFv is present only in traces. We have suspects that pelB-Ab is toxic for this E. coli strain.Chassis
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