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Team:Technion
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Viruses can be described as complex 3D structures capable of efficient infection of their target organism. Because of their highly specific infection ability, they can be used as vessels for "smart" therapeutic strategies which rely on an agent that can effectively analyze the cellular environment and compute an appropriate response. To demonstrate the potential of a "smart" strategy, we are developing a "Trojan Horse" type of approach based on bactriophage-lambda.<br> | Viruses can be described as complex 3D structures capable of efficient infection of their target organism. Because of their highly specific infection ability, they can be used as vessels for "smart" therapeutic strategies which rely on an agent that can effectively analyze the cellular environment and compute an appropriate response. To demonstrate the potential of a "smart" strategy, we are developing a "Trojan Horse" type of approach based on bactriophage-lambda.<br> | ||
Our project uses phage lambda and its target organism, E.coli, as a proof of concept for creating a system with predefined actions that demonstrates the described strategy. The design is based on a high specificity system which combines several different cell elements that will function as a type of logic AND gate. The phage will not harm the bacteria unless three independent conditions are met, activating the phage's lytic cycle and resulting in the bacteria's death; imitating a "Trojan Horse".<br> | Our project uses phage lambda and its target organism, E.coli, as a proof of concept for creating a system with predefined actions that demonstrates the described strategy. The design is based on a high specificity system which combines several different cell elements that will function as a type of logic AND gate. The phage will not harm the bacteria unless three independent conditions are met, activating the phage's lytic cycle and resulting in the bacteria's death; imitating a "Trojan Horse".<br> | ||
| - | Read more about our project on the [https://2012.igem.org/Team:Technion/Project/Description project description] | + | Read more about our project on the [https://2012.igem.org/Team:Technion/Project/Description project description] and [https://2012.igem.org/Team:Technion/Project/Overview project overview] pages. |
==The reporter system standard== | ==The reporter system standard== | ||
One of our main achievements was a reporter system standard. This standard was used to measure the expression of phage RNA polymerases (such as T7 RNA polymerase), which were an intermediate product in our system. The reporter system is composed of phage promoters followed by one of two reporter genes: alkaline phosphatase and <em>xylE</em>. These reporters expand the variety of reporter proteins beyond the commonly used fluorescent proteins, <em>lacZ</em> and luciferase. Read more about this standard [https://2012.igem.org/Team:Technion/Project/Reporter here]. | One of our main achievements was a reporter system standard. This standard was used to measure the expression of phage RNA polymerases (such as T7 RNA polymerase), which were an intermediate product in our system. The reporter system is composed of phage promoters followed by one of two reporter genes: alkaline phosphatase and <em>xylE</em>. These reporters expand the variety of reporter proteins beyond the commonly used fluorescent proteins, <em>lacZ</em> and luciferase. Read more about this standard [https://2012.igem.org/Team:Technion/Project/Reporter here]. | ||
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Our project, in short
Viruses can be described as complex 3D structures capable of efficient infection of their target organism. Because of their highly specific infection ability, they can be used as vessels for "smart" therapeutic strategies which rely on an agent that can effectively analyze the cellular environment and compute an appropriate response. To demonstrate the potential of a "smart" strategy, we are developing a "Trojan Horse" type of approach based on bactriophage-lambda.
Our project uses phage lambda and its target organism, E.coli, as a proof of concept for creating a system with predefined actions that demonstrates the described strategy. The design is based on a high specificity system which combines several different cell elements that will function as a type of logic AND gate. The phage will not harm the bacteria unless three independent conditions are met, activating the phage's lytic cycle and resulting in the bacteria's death; imitating a "Trojan Horse".
Read more about our project on the project description and project overview pages.
The reporter system standard
One of our main achievements was a reporter system standard. This standard was used to measure the expression of phage RNA polymerases (such as T7 RNA polymerase), which were an intermediate product in our system. The reporter system is composed of phage promoters followed by one of two reporter genes: alkaline phosphatase and xylE. These reporters expand the variety of reporter proteins beyond the commonly used fluorescent proteins, lacZ and luciferase. Read more about this standard here.
