Team:Carnegie Mellon/Bio-Submitted

From 2012.igem.org

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Mutant III: Average <br>
Mutant III: Average <br>
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RBS used in construct is: CATATG <b>AAGAAGGAGA</b> TATACC
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Revision as of 07:00, 24 September 2012

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Submitted Parts

BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA

Hypothesis:

Expected promoter strength of different mutants:
Mutant I: <100%
Mutant II: ~100%
Mutant III: ~50%

Expected LacI leaky expression of different mutants:
Mutant I: More than average
Mutant II: Average
Mutant III: Average

RBS used in construct is: CATATG AAGAAGGAGA TATACC

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