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Team:Trieste/notebook10
From 2012.igem.org
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<div id="suicide" class="notebook_section"> | <div id="suicide" class="notebook_section"> | ||
<h2 class="notebook_title">Suicide System</h2> | <h2 class="notebook_title">Suicide System</h2> | ||
| - | We transformed also the double terminator (BBa_B0015) as Bglbrick. We amplified by PCR the | + | We transformed also the double terminator (BBa_B0015) as Bglbrick. We amplified by PCR the BBa_B0015 miniprep with the appropriate PCR protocol. We loaded the gel with the amplified and the eluted was ligated in the pGEM vector.</br> |
</br> | </br> | ||
The plasmid was amplified with the primers SP6 and T7 and then cut BglII/XhoI.</br> | The plasmid was amplified with the primers SP6 and T7 and then cut BglII/XhoI.</br> | ||
</br> | </br> | ||
| - | For the final construct | + | For the final construct -holin LL 37- :the LL 37 (BBa_K875009) was cloned downstream the T5CumateOperator (BBa_K875001), heat inactivated and dephosphorylated at the extremities. |
</div> | </div> | ||
<div id="antibody" class="notebook_section"> | <div id="antibody" class="notebook_section"> | ||
Revision as of 19:26, 23 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Week 10
More
Suicide System
We transformed also the double terminator (BBa_B0015) as Bglbrick. We amplified by PCR the BBa_B0015 miniprep with the appropriate PCR protocol. We loaded the gel with the amplified and the eluted was ligated in the pGEM vector. The plasmid was amplified with the primers SP6 and T7 and then cut BglII/XhoI. For the final construct -holin LL 37- :the LL 37 (BBa_K875009) was cloned downstream the T5CumateOperator (BBa_K875001), heat inactivated and dephosphorylated at the extremities.Antibody
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Chassis
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
