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Team:Austin Texas/Plasmids and Strains
From 2012.igem.org
(Difference between revisions)
(→Strain Database) |
(→Plasmid Database) |
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| Line 11: | Line 11: | ||
! Plasmid | ! Plasmid | ||
! Source | ! Source | ||
| + | ! Added by | ||
|- | |- | ||
| - | | | + | | Plasmid Box 1 |
| 2 | | 2 | ||
| | | | ||
| Line 19: | Line 20: | ||
| ASKA cadA plasmid | | ASKA cadA plasmid | ||
| Barrick Lab | | Barrick Lab | ||
| + | | | ||
|- | |- | ||
| - | | | + | | Plasmid Box 1 |
| 3 | | 3 | ||
| | | | ||
| Line 27: | Line 29: | ||
| ASKA ldcC plasmid | | ASKA ldcC plasmid | ||
| Barrick Lab | | Barrick Lab | ||
| + | | | ||
|- | |- | ||
| - | | | + | | Plasmid Box 1 |
| | | | ||
| IGM004 | | IGM004 | ||
| Line 35: | Line 38: | ||
| pSB3K3 | | pSB3K3 | ||
| 2012 kit plate 2 (15L) | | 2012 kit plate 2 (15L) | ||
| + | | Ben | ||
|- | |- | ||
| - | | | + | | Plasmid Box 1 |
| | | | ||
| IGM005 | | IGM005 | ||
| Line 43: | Line 47: | ||
| pSB1AK3 | | pSB1AK3 | ||
| 2012 kit plate 3 (12O) | | 2012 kit plate 3 (12O) | ||
| + | | Ben | ||
|- | |- | ||
| - | | | + | | Plasmid Box 1 |
| | | | ||
| IGM006 | | IGM006 | ||
| Line 51: | Line 56: | ||
| pSB1A2 | | pSB1A2 | ||
| 2012 kit plate 2 (10N) | | 2012 kit plate 2 (10N) | ||
| + | | Ben | ||
|- | |- | ||
| - | | | + | | Plasmid Box 1 |
| | | | ||
| IGM007 | | IGM007 | ||
| Line 59: | Line 65: | ||
| pSB1A2 | | pSB1A2 | ||
| 2012 kit plate 1 (7L) | | 2012 kit plate 1 (7L) | ||
| + | | Aurko | ||
|- | |- | ||
| - | | | + | | Plasmid Box 1 |
| | | | ||
| IGM008 | | IGM008 | ||
| - | | Double terminator (BBa_B0010-BBa_B0012) | + | | Double terminator (BBa_B0010 - BBa_B0012) |
| [http://www.partsregistry.org/Part:BBa_B0015 BBa_B0015] | | [http://www.partsregistry.org/Part:BBa_B0015 BBa_B0015] | ||
| pSB1AK3 | | pSB1AK3 | ||
| 2012 kit plate 1 (23L) | | 2012 kit plate 1 (23L) | ||
| + | | Aurko | ||
|- | |- | ||
| - | | | + | | Plasmid Box 1 |
| | | | ||
| IGM009 | | IGM009 | ||
| Line 75: | Line 83: | ||
| pSB1C3 | | pSB1C3 | ||
| 2012 kit plate 5 (1K) | | 2012 kit plate 5 (1K) | ||
| + | | | ||
|} | |} | ||
Revision as of 21:27, 19 June 2012
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
| Box | Slot | Label | Name | Part | Plasmid | Source | Added by |
|---|---|---|---|---|---|---|---|
| Plasmid Box 1 | 2 | cadA | ASKA cadA plasmid | Barrick Lab | |||
| Plasmid Box 1 | 3 | ldcC | ASKA ldcC plasmid | Barrick Lab | |||
| Plasmid Box 1 | IGM004 | GFP | [http://www.partsregistry.org/Part:BBa_I714891 BBa_I714891] | pSB3K3 | 2012 kit plate 2 (15L) | Ben | |
| Plasmid Box 1 | IGM005 | RFP | [http://www.partsregistry.org/Part:BBa_K081014 BBa_K081014] | pSB1AK3 | 2012 kit plate 3 (12O) | Ben | |
| Plasmid Box 1 | IGM006 | LasI | [http://www.partsregistry.org/Part:BBa_K081009 BBa_K081009] | pSB1A2 | 2012 kit plate 2 (10N) | Ben | |
| Plasmid Box 1 | IGM007 | RBS + LacI | [http://www.partsregistry.org/Part:BBa_J24679 BBa_J24679] | pSB1A2 | 2012 kit plate 1 (7L) | Aurko | |
| Plasmid Box 1 | IGM008 | Double terminator (BBa_B0010 - BBa_B0012) | [http://www.partsregistry.org/Part:BBa_B0015 BBa_B0015] | pSB1AK3 | 2012 kit plate 1 (23L) | Aurko | |
| Plasmid Box 1 | IGM009 | J23100 promoter - sfGFP (Part:BBa_K515105) | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105] | pSB1C3 | 2012 kit plate 5 (1K) |
Strain Database
All strains are in the back right rack of the –80°C freezer.
| Box | Slot | Label | Description | Strain | Source | Added by |
|---|---|---|---|---|---|---|
| iGEM 2012 Box 1 | 2 | AG1 | Host strain of ASKA collection | Barrick Lab | ||
| iGEM 2012 Box 1 | 3 | BW25113 | Host strain of Keio collection | Barrick Lab | ||
| iGEM 2012 Box 1 | 4 | cadA | AG1 + ASKA collection cadA plasmid, CamR | Barrick Lab | ||
| iGEM 2012 Box 1 | 5 | ldcC | AG1 + ASKA collection ldcC plasmid, CamR | Barrick Lab | ||
| iGEM 2012 Box 1 | 6 | tnaA | Keio collection knockout (version of odor-less E. coli), KanR | Barrick Lab | ||
| iGEM 2012 Box 1 | 7 | IGM009 | crdASC (4864 bp) | TOP10 | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 8 | IGM010 | crdASC (4943 bp) | TOP10 | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 9 | IGM011 | crdASC (4864 bp) | BL21(DE3) | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 10 | IGM012 | crdASC (4943 bp) | BL21(DE3) | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 11 | IGM013 | BBa_K515105: J23100-sfGFP-pSB1C3(CamR) | epi300 | Parts Kit |
ZombiE.coli parts
| Plasmid | Part | Description | Source |
|---|---|---|---|
| pReporter | [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] | GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) | 2012 kit plate 2 (15L) |
| pReporter | [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] | RFP protein generator - PoPS->RFP | 2012 kit plate 3 (12O) |
| pReporter | [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] | LasI protein generator (TERM-) | 2012 kit plate 2 (10N) |
| pReporter | [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | RBS + LacI | 2012 kit plate 1 (7L) |
| pReporter | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | Double terminator (BBa_B0010-BBa_B0012) | 2012 kit plate 1 (23L) |
| pConverter | [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] | LasR Promoter | 2012 kit plate 1 (12A) |
| pConverter | [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] | LasR | 2012 kit plate 1 (14J) |
| pConverter | [http://partsregistry.org/Part:BBa_K082016 BBa_K082016] | LacIM1 | Parts Registry |
| pConverter | [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | LacI Promoter | 2012 kit plate 1 (1D) |
| pConverter | CRE | pQL269 Plasmid |
