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Team:Trieste/notebook7
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<div id="suicide" class="notebook_section"> | <div id="suicide" class="notebook_section"> | ||
<h2 class="notebook_title">Suicide System</h2> | <h2 class="notebook_title">Suicide System</h2> | ||
| - | We received the sequencing results of RBS_B0031-Tse2-TT_B0015 construct and double checked before the BB submission. At this point we were able to clone it downstream the T5LacOperator Promoter(BBa_K875002)to test its functionality. We screened the colonies and two of them resulted positive (T5LacOp-B0031-Tse2-B0015 ), so we plated them with and without IPTG. One of the two colonies doesn't grow on IPTG and grew without it! We also did the same test in LB culture and the same colony died with IPTG and grew without it.</br> | + | We received the sequencing results of RBS_B0031-Tse2-TT_B0015 construct and double checked before the BB submission. At this point we were able to clone it downstream the T5LacOperator Promoter (BBa_K875002) to test its functionality. We screened the colonies and two of them resulted positive (T5LacOp-B0031-Tse2-B0015), so we plated them with and without IPTG. One of the two colonies doesn't grow on IPTG and grew without it! We also did the same test in LB culture and the same colony died with IPTG and grew without it.</br> |
</br> | </br> | ||
We also recorded the OD of the LB culture IPTG induced and not induced at fixed time intervals for several hours during the day. We designed the growth curve. | We also recorded the OD of the LB culture IPTG induced and not induced at fixed time intervals for several hours during the day. We designed the growth curve. | ||
Revision as of 17:23, 23 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Week 7
More
Suicide System
We received the sequencing results of RBS_B0031-Tse2-TT_B0015 construct and double checked before the BB submission. At this point we were able to clone it downstream the T5LacOperator Promoter (BBa_K875002) to test its functionality. We screened the colonies and two of them resulted positive (T5LacOp-B0031-Tse2-B0015), so we plated them with and without IPTG. One of the two colonies doesn't grow on IPTG and grew without it! We also did the same test in LB culture and the same colony died with IPTG and grew without it. We also recorded the OD of the LB culture IPTG induced and not induced at fixed time intervals for several hours during the day. We designed the growth curve.Antibody
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Chassis
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
