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Team:Trieste/notebook4
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<div id="suicide" class="notebook_section"> | <div id="suicide" class="notebook_section"> | ||
<h2 class="notebook_title">Suicide System</h2> | <h2 class="notebook_title">Suicide System</h2> | ||
| - | + | Due to the negative results of the previous week , we decided to use another toxin from the registry, that we have already extracted from iGEM kit and amplified it through the transformation of DH5-alpha competent cells: Tse2 toxin (BB_K314200).</br> | |
| + | </br> | ||
| + | We began to create our toxin construct, cutting Tse2 Toxin (EcoRI/SpeI) to clone it inside the double terminator BB_B0015, previously cut with EcoRI/XbaI.</br> | ||
| + | </br> | ||
| + | Unfortunately we did not obtain a positive colony, so we tried different cloning approaches. | ||
</div> | </div> | ||
<div id="antibody" class="notebook_section"> | <div id="antibody" class="notebook_section"> | ||
Revision as of 16:45, 23 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Week 4
More
Suicide System
Due to the negative results of the previous week , we decided to use another toxin from the registry, that we have already extracted from iGEM kit and amplified it through the transformation of DH5-alpha competent cells: Tse2 toxin (BB_K314200). We began to create our toxin construct, cutting Tse2 Toxin (EcoRI/SpeI) to clone it inside the double terminator BB_B0015, previously cut with EcoRI/XbaI. Unfortunately we did not obtain a positive colony, so we tried different cloning approaches.Antibody
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Chassis
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
