Team:EPF-Lausanne/Parts

From 2012.igem.org

(Difference between revisions)
Line 3: Line 3:
{{:Team:EPF-Lausanne/Template/SetTitle|Parts Submitted to the Registry}}
{{:Team:EPF-Lausanne/Template/SetTitle|Parts Submitted to the Registry}}
 +
=Our parts=
-
An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
+
We managed to submit 4 new parts that will give other teams the possibility to reproduce our experiments with both the switches we tested.
-
Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
+
The melanopsin coding gene and the NFAT response element will allow to use the more complex switch, by just cloning melanopsin into a mammalian vector and clone a readout gene (fluorescent protein, quantitatively measurable protein) downstream NFAT and clone both in a mammalian vector.
 +
 
 +
We also submitted our favorite parts, which are a mammalian LovTAP-VP16 and a composite readout system build to respond to it.
 +
Both parts only need cloning into a mammalian vector to be ready for transfection.

Revision as of 15:28, 23 September 2012

Our parts

We managed to submit 4 new parts that will give other teams the possibility to reproduce our experiments with both the switches we tested.

The melanopsin coding gene and the NFAT response element will allow to use the more complex switch, by just cloning melanopsin into a mammalian vector and clone a readout gene (fluorescent protein, quantitatively measurable protein) downstream NFAT and clone both in a mammalian vector.

We also submitted our favorite parts, which are a mammalian LovTAP-VP16 and a composite readout system build to respond to it. Both parts only need cloning into a mammalian vector to be ready for transfection.


<groupparts>iGEM012 EPF-Lausanne</groupparts>