Team:XMU-China/protocolsindex

From 2012.igem.org

(Difference between revisions)

Revision as of 14:43, 23 September 2012

safetyindex

Contents[hide] [show]
1 General protocols
1.1 Stock solution
1.2 Preparation of Competent BL21
1.3 Transformation
1.4 Plasmid Purification
1.5 Reaction system of restriction endonuclease
1.6 Standard BioBrick Assembly
1.7 Suffix Insertion
1.8 Ligation
1.9 Restriction analysis
1.10 Gel Extraction
2 Characterization
2.1 Fluorescence Measurements
2.2 Protein electrophoresis
2.2.1 Preparation of Samples
2.2.2 Manufacture Albumen Gel
2.2.3 Electrophoresis
2.2.4 Dyeing (Colloidal Coomassie Brilliant Blue)
2.2.5 Scanning
3 Immobilization
3.1 Prepare Sodiumcellu-losesulfate (NaCS)
3.2 Prepare microcapsules
3.3 Immobilize cells into calcium alginate beads
3.4 Immobilize cells into intra-hollow Ca-alginate capsules
3.5 Immobilize cells into NaCS-PDMDAAC microcapsules

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-       <th>Contents<span id="hide"><a href="#" onClick="MM_changeProp('indextd4','','display','none','TD');MM_changeProp('indextd1','','display','none','TD');MM_changeProp('indextd2','','display','none','TD');MM_changeProp('indextd3','','display','none','TD');MM_changeProp('hide','','display','none','SPAN');MM_changeProp('indextd5','','display','none','TD');MM_changeProp('indextd6','','display','none','TD');MM_changeProp('indextd7','','display','none','TD');MM_changeProp('indextd8','','display','none','TD');MM_changeProp('indextd9','','display','none','TD');MM_changeProp('indextd10','','display','none','TD');MM_changeProp('indextd11','','display','none','TD');MM_changeProp('indextd12','','display','none','TD');MM_changeProp('indextd13','','display','none','TD');MM_changeProp('indextd14','','display','none','TD');MM_changeProp('indextd15','','display','none','TD');MM_changeProp('show','','display','block','SPAN');MM_changeProp('indextd16','','display','none','TD')">[hide]</a></span>
+       <th>Contents<span id="hide"><a href="#" onClick="MM_changeProp('indextd4','','display','none','TD');MM_changeProp('indextd1','','display','none','TD');MM_changeProp('indextd2','','display','none','TD');MM_changeProp('indextd3','','display','none','TD');MM_changeProp('hide','','display','none','SPAN');MM_changeProp('indextd5','','display','none','TD');MM_changeProp('indextd6','','display','none','TD');MM_changeProp('indextd7','','display','none','TD');MM_changeProp('indextd8','','display','none','TD');MM_changeProp('indextd9','','display','none','TD');MM_changeProp('indextd10','','display','none','TD');MM_changeProp('indextd11','','display','none','TD');MM_changeProp('indextd12','','display','none','TD');MM_changeProp('indextd13','','display','none','TD');MM_changeProp('indextd14','','display','none','TD');MM_changeProp('indextd15','','display','none','TD');MM_changeProp('show','','display','block','SPAN');MM_changeProp('indextd16','','display','none','TD');MM_changeProp('indextd17','','display','none','TD');MM_changeProp('indextd18','','display','none','TD');MM_changeProp('indextd19','','display','none','TD');MM_changeProp('indextd20','','display','none','TD');MM_changeProp('indextd21','','display','none','TD');MM_changeProp('indextd22','','display','none','TD');MM_changeProp('indextd23','','display','none','TD');MM_changeProp('indextd24','','display','none','TD');MM_changeProp('indextd25','','display','none','TD')">[hide]</a></span>
-   <span id="show"><a href="#" onClick="MM_changeProp('hide','','display','block','SPAN');MM_changeProp('show','','display','none','SPAN');MM_changeProp('indextd1','','display','block','TD');MM_changeProp('indextd2','','display','block','TD');MM_changeProp('indextd3','','display','block','TD');MM_changeProp('indextd4','','display','block','TD');MM_changeProp('indextd5','','display','block','TD');MM_changeProp('indextd6','','display','block','TD');MM_changeProp('indextd7','','display','block','TD');MM_changeProp('indextd8','','display','block','TD');MM_changeProp('indextd9','','display','block','TD');MM_changeProp('indextd10','','display','block','TD');MM_changeProp('indextd11','','display','block','TD');MM_changeProp('indextd12','','display','block','TD');MM_changeProp('indextd13','','display','block','TD');MM_changeProp('indextd14','','display','block','TD');MM_changeProp('indextd15','','display','block','TD');MM_changeProp('indextd16','','display','block','TD')">[show]</a></span></th>
+   <span id="show"><a href="#" onClick="MM_changeProp('hide','','display','block','SPAN');MM_changeProp('show','','display','none','SPAN');MM_changeProp('indextd1','','display','block','TD');MM_changeProp('indextd2','','display','block','TD');MM_changeProp('indextd3','','display','block','TD');MM_changeProp('indextd4','','display','block','TD');MM_changeProp('indextd5','','display','block','TD');MM_changeProp('indextd6','','display','block','TD');MM_changeProp('indextd7','','display','block','TD');MM_changeProp('indextd8','','display','block','TD');MM_changeProp('indextd9','','display','block','TD');MM_changeProp('indextd10','','display','block','TD');MM_changeProp('indextd11','','display','block','TD');MM_changeProp('indextd12','','display','block','TD');MM_changeProp('indextd13','','display','block','TD');MM_changeProp('indextd14','','display','block','TD');MM_changeProp('indextd15','','display','block','TD');MM_changeProp('indextd16','','display','block','TD');MM_changeProp('indextd17','','display','block','TD');MM_changeProp('indextd18','','display','block','TD');MM_changeProp('indextd19','','display','block','TD');MM_changeProp('indextd20','','display','block','TD');MM_changeProp('indextd21','','display','block','TD');MM_changeProp('indextd22','','display','block','TD');MM_changeProp('indextd23','','display','block','TD');MM_changeProp('indextd24','','display','block','TD');MM_changeProp('indextd25','','display','block','TD')">[show]</a></span></th>
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-       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc17"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.3&nbsp;Electrophoresis</strong></a></li> </td>
+       <td id="indextd17"><li id="indexli"><a id="indexa" href="#_Toc17"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.3&nbsp;Electrophoresis</strong></a></li> </td>
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-       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc18"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.4&nbsp;Dyeing (Colloidal Coomassie Brilliant Blue)</strong></a></li> </td>
+       <td id="indextd18"><li id="indexli"><a id="indexa" href="#_Toc18"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.4&nbsp;Dyeing (Colloidal Coomassie Brilliant Blue)</strong></a></li> </td>
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-       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc19"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.5&nbsp;Scanning</strong></a></li> </td>
+       <td id="indextd19"><li id="indexli"><a id="indexa" href="#_Toc19"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.5&nbsp;Scanning</strong></a></li> </td>
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-       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc20"><strong>3 Immobilization</strong></a></li> </td>
+       <td id="indextd20"><li id="indexli"><a id="indexa" href="#_Toc20"><strong>3 Immobilization</strong></a></li> </td>
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-       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc21"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.1&nbsp;Prepare Sodiumcellu-losesulfate (NaCS)</strong></a></li> </td>
+       <td id="indextd21"><li id="indexli"><a id="indexa" href="#_Toc21"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.1&nbsp;Prepare Sodiumcellu-losesulfate (NaCS)</strong></a></li> </td>
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-       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc22"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.2&nbsp;Prepare microcapsules</strong></a></li> </td>
+       <td id="indextd22"><li id="indexli"><a id="indexa" href="#_Toc22"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.2&nbsp;Prepare microcapsules</strong></a></li> </td>
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-       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc23"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.3&nbsp;Immobilize cells into calcium alginate beads</strong></a></li> </td>
+       <td id="indextd23"><li id="indexli"><a id="indexa" href="#_Toc23"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.3&nbsp;Immobilize cells into calcium alginate beads</strong></a></li> </td>
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-       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc24"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.4&nbsp;Immobilize cells into intra-hollow Ca-alginate capsules</strong></a></li> </td>
+       <td id="indextd24"><li id="indexli"><a id="indexa" href="#_Toc24"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.4&nbsp;Immobilize cells into intra-hollow Ca-alginate capsules</strong></a></li> </td>
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-       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc25"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.5&nbsp;Immobilize cells into NaCS-PDMDAAC microcapsules</strong></a></li> </td>
+       <td id="indextd25"><li id="indexli"><a id="indexa" href="#_Toc25"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.5&nbsp;Immobilize cells into NaCS-PDMDAAC microcapsules</strong></a></li> </td>
      </tr>